Immunoprecipitation and western blot analysis were performed as previously described (Sayeski et al., 1998 (link)). To determine phosphorylation of p47phox, protein sample was extracted from the homogenates of isolated DCs and immunoprecipitated using p47phox antibody (Millipore) followed by western blotting using phospho-p47phox antibody (Sigma). To investigate association of p47phox with gp91phox, protein lysates from DCs were immunoprecipitated using the gp91phox antibody (Becton Dickinson) followed by western blotting using phospho-p47phox antibody (Millipore).
P47phox antibody
Manufactured by Merck Group
The P47phox antibody is a laboratory tool used in research applications. It is designed to detect and bind to the P47phox protein, which is a component of the NADPH oxidase complex. The P47phox antibody can be utilized in various immunoassay techniques to study the expression and localization of the P47phox protein in biological samples.
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Lab products found in correlation
2 protocols using p47phox antibody
1
Phosphorylation and Association of p47phox
2
Phosphorylation and Association of p47phox
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