Primary mouse hepatic stellate cells (HSCs) were isolated by collagenase/pronase digestion and Stractan density gradient centrifugation before being cultured on plastic for 3 days with or without L4F-A192 (Table 1 ) at 37 °C [3 (link)]. HSCs were fixed with 10% formaldehyde. Primary antibody staining was done using a mouse monoclonal anti-α-SMA antibody (1:100, Sigma), followed by a fluorescein isothiocyanate-conjugated goat anti-mouse secondary antibody (Sigma). Images were acquired using a Nikon PCM-2000 confocal microscope with a thickness of 0.8 μm.
Fluorescein isothiocyanate conjugated goat anti mouse secondary antibody
Manufactured by Merck Group
Fluorescein isothiocyanate-conjugated goat anti-mouse secondary antibody is a laboratory reagent used in immunoassays and immunohistochemistry. It consists of a goat-derived secondary antibody that is conjugated to the fluorescent dye fluorescein isothiocyanate (FITC). This product can be used to detect and visualize primary mouse antibodies in various experimental applications.
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2 protocols using fluorescein isothiocyanate conjugated goat anti mouse secondary antibody
1
Isolation and Activation of Primary Mouse Hepatic Stellate Cells
2
Immunofluorescence Analysis of Recombinant Bovine Enterovirus
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The rescued recombinant virus rBEV-E0 and parental virus were transfected in MDBK cells in 96-well plates. After 24 h, the cells were fixed with ice-cold anhydrous ethanol at 4 °C for 30 min, followed by the addition of 0.3% Triton 100 for 15 min. Anti-BEV VP1 mAb and anti-BVDV E0 PcAb (1:1000) were added and the cells were further incubated at 37 °C for 1 h, followed by incubation with fluorescein isothiocyanate-conjugated goat anti-mouse secondary antibody (1:500; Sigma-Aldrich) in the dark. The results were observed under a fluorescence microscope.
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