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Infinity alt liquid stable reagent

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The Infinity ALT Liquid Stable Reagent is a laboratory equipment designed for the measurement of alanine aminotransferase (ALT) levels in biological samples. It is a liquid-stable formulation that provides consistent and reliable results.

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Lab products found in correlation

Microcon centrifugal filter device, by Merck Group (2 mentions) Power wave x spectrophotometer, by Agilent Technologies (1 mentions) Spectramax id3 microplate reader, by Molecular Devices (1 mentions) Spectramax id3, by Molecular Devices (1 mentions)

Spelling variants of this product

Infinity reagents (72 citations) Infinity ALT (10 citations) Infinity ALT Reagent (9 citations) Infinity ALT (GPT) Liquid Stable Reagent (5 citations) Infinity™ ALT and AST Liquid Stable Reagent (4 citations)

11 protocols using infinity alt liquid stable reagent

1

Serum ALT and Liver Triglycerides

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Serum alanine aminotransferase (ALT) levels were assessed as a measure of liver damage by using the Infinity ALT liquid stable reagent (Thermo Electron, Waltham, MA). Triglycerides were extracted from liver homogenates with chloroform/methanol (2:1 v/v) and triglyceride concentration assayed using commercially available reagents (Cayman Chemical, Ann Arbor, MI).
Shearn C.T., Pulliam C.F., Pedersen K., Meredith K., Mercer K.E., Saba L.M., Orlicky D.J., Ronis M.J, & Petersen D.R. (2018). Knock-out of the Gsta4 gene in male mice leads to an altered pattern of hepatic protein carbonylation and enhanced inflammation following chronic consumption of an ethanol diet. Alcoholism, clinical and experimental research, 42(7), 1192-1205.
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2

Hepatic Steatosis and Oxidative Stress

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Hepatic steatosis was measured by image analysis of Oil Red O staining and biochemically using Triglyceride Reagent (Synermed). Liver lipid peroxidation (thiobarbituric acid reactive substances, TBARS) was assessed as a measure of oxidative stress as described by Ohkawa, Ohishi, & Yagi (1979). Serum alanine amino transferase activities (ALTs) were measured to assess liver necrosis using the Infinity™ ALT liquid stable reagent (Thermo Electron Corp., Waltham, MA) according to manufacturer’s protocols.
Ronis M.J., Mercer K., Suva L.J., Vantrease J., Ferguson M., Hogue W.R., Sharma N., Cleves M.A., Blackburn M.L, & Badger T.M. (2014). Influence of Fat/Carbohydrate Ratio on Progression of Fatty Liver Disease and on Development of Osteopenia in Male Rats Fed Alcohol via Total Enteral Nutrition (TEN). Alcohol (Fayetteville, N.Y.), 48(2), 133-144.
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3

Spectrophotometric ALT Enzyme Assay

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Plasma or serum ALT activity was determined as a biochemical indicator of hepatocellular injury. Infinity ALT Liquid Stable Reagent (Thermo Fisher Scientific Inc., Waltham, MA) was used to determine ALT activity. Briefly, 100 μL of reagent was added to 10 μL serum or plasma samples, and absorbance was measured spectrophotometrically at 340 nmusing a Bio-Tek Power Wave X Spectrophotometer. ALT activity (IU/L) was determined using the molar extinction coefficient of NADH (6.3 mM−1cm−1).
Rudraiah S., Moscovitz J.E., Donepudi A.C., Campion S.N., Slitt A.L., Aleksunes L.M, & Manautou J.E. (2014). Differential Fmo3 Gene Expression in Various Liver Injury Models Involving Hepatic Oxidative Stress in Mice. Toxicology, 325, 85-95.
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4

Serum ALT Enzyme Quantification

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The presence of ALT enzyme in the serum was detected using Infinity ALT Liquid Stable Reagent (Thermo Scientific) with Enzyme ER Verifier Kit (Verichem Laboratories) standards, and analyzed using a SpectraMax iD3 microplate reader (Molecular Devices).
Chiale C., Moshkani S., Rose J.K, & Robek M.D. (2019). Heterologous prime-boost immunization with vesiculovirus-based vectors expressing HBV Core antigen induces CD8+ T cell responses in naïve and persistently infected mice and protects from challenge. Antiviral research, 168, 156-167.
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5

Hepatic Ischemia-Reperfusion Injury in Mice

Cited 1 time
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Mice were anesthetized, injected with heparin (100 U/kg), and an atraumatic clip was used to interrupt the hepatic artery/portal venous blood supply to the left/middle liver lobes.25 (link) After 60 min of partial (75%) warm ischemia, the clamp was removed, and mice were sacrificed at 6 h of reperfusion. The sALT levels were measured with Infinity ALT Liquid Stable Reagent (Thermo Scientific, Rockford, IL).
To focus on macrophage-specific Ikaros function, CD11b-DTR mice were first treated with diphtheria toxin (DT; 25 ng/g i.v. at day −1) to deplete native CD11b+ cells,26 (link) and then infused with in vitro generated siRNA-Ikaros vs. siRNA-Control macrophages (5×105 cells i.v.) at 1 h before hepatic IR-insult. DT treatment does not trigger hepatotoxicity in WT mice.26 (link)For further details regarding the materials and methods used, please refer to the CTAT table and supplementary information.
Kadono K., Kageyama S., Nakamura K., Hirao H., Ito T., Kojima H., Dery K.J., Li X, & Kupiec-Weglinski J.W. (2021). Myeloid Ikaros–SIRT1 signaling axis regulates hepatic inflammation and pyroptosis in ischemia-stressed mouse and human liver. Journal of hepatology, 76(4), 896-909.
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6

Serum Biomarker Quantification for Hepatocyte Injury

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For the animal experiments, immediately after euthanasia, we collected systemic blood from the inferior vena cava. Serum was obtained by centrifugation of whole blood at 10,000 rpm for 10 minutes. For the in vitro experiments, cell-free culture supernatant was concentrated to 500 μL using Microcon centrifugal filter devices (Millipore, Billerica, MA). To determine the extent of hepatocyte injury, quantification of alanine aminotransferase (ALT) was performed from serum and concentrated culture supernatant using the Infinity ALT Liquid Stable Reagent (Thermo Scientific, Middletown, VA).
Luther J., Garber J.J., Khalili H., Dave M., Bale S.S., Jindal R., Motola D.L., Luther S., Bohr S., Jeoung S.W., Deshpande V., Singh G., Turner J.R., Yarmush M.L., Chung R.T, & Patel S.J. (2015). Hepatic Injury in Nonalcoholic Steatohepatitis Contributes to Altered Intestinal Permeability. Cellular and Molecular Gastroenterology and Hepatology, 1(2), 222-232.e2.
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7

Quantifying Hepatocyte Injury: ALT Assay

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For the animal experiments, immediately after euthanasia, we collected systemic blood from the inferior vena cava. Serum was obtained by centrifugation of whole blood at 10,000 rpm for 10 minutes. For the in vitro experiments, cell-free culture supernatant was concentrated to 500 μL using Microcon centrifugal filter devices (Millipore, Billerica, MA). To determine the extent of hepatocyte injury, quantification of alanine aminotransferase (ALT) was performed from serum and concentrated culture supernatant using the Infinity ALT Liquid Stable Reagent (Thermo Scientific, Middletown, VA).
Luther J., Garber J.J., Khalili H., Dave M., Bale S.S., Jindal R., Motola D.L., Luther S., Bohr S., Jeoung S.W., Deshpande V., Singh G., Turner J.R., Yarmush M.L., Chung R.T, & Patel S.J. (2015). Hepatic Injury in Nonalcoholic Steatohepatitis Contributes to Altered Intestinal Permeability. Cellular and Molecular Gastroenterology and Hepatology, 1(2), 222-232.e2.
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8

Serum ALT Measurement Protocol

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Serum ALT levels were measured using Infinity ALT Liquid Stable Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions.
Forootan S.S., Mutter F.E., Kipar A., Iwawaki T., Francis B., Goldring C.E., Park B.K, & Copple I.M. (2017). Real-time in vivo imaging reveals localised Nrf2 stress responses associated with direct and metabolism-dependent drug toxicity. Scientific Reports, 7, 16084.
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9

Hepatic Ischemia-Reperfusion Injury in Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were anesthetized, injected with heparin (100 U/kg), and an atraumatic clip was used to interrupt the hepatic artery/portal venous blood supply to the left/middle liver lobes.25 (link) After 60 min of partial (75%) warm ischemia, the clamp was removed, and mice were sacrificed at 6 h of reperfusion. The sALT levels were measured with Infinity ALT Liquid Stable Reagent (Thermo Scientific, Rockford, IL).
To focus on macrophage-specific Ikaros function, CD11b-DTR mice were first treated with diphtheria toxin (DT; 25 ng/g i.v. at day −1) to deplete native CD11b+ cells,26 (link) and then infused with in vitro generated siRNA-Ikaros vs. siRNA-Control macrophages (5×105 cells i.v.) at 1 h before hepatic IR-insult. DT treatment does not trigger hepatotoxicity in WT mice.26 (link)For further details regarding the materials and methods used, please refer to the CTAT table and supplementary information.
Kadono K., Kageyama S., Nakamura K., Hirao H., Ito T., Kojima H., Dery K.J., Li X, & Kupiec-Weglinski J.W. (2021). Myeloid Ikaros–SIRT1 signaling axis regulates hepatic inflammation and pyroptosis in ischemia-stressed mouse and human liver. Journal of hepatology, 76(4), 896-909.
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10

Quantification of Serum ALT Levels

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Isolated baseline and terminal serum was utilized for analysis of ALT. Concentrations were quantified using the Infinity ALT Liquid Stable Reagent (Thermo Scientific, Hudson, NH) on a SpectraMax microtiter plate reader (Molecular Devices, Sunnyvale, CA) following the manufacturer’s protocol.
Church R.J., Gatti D.M., Urban T.J., Long N., Yang X., Shi Q., Eaddy J.S., Mosedale M., Ballard S., Churchill G.A., Navarro V., Watkins P.B., Threadgill D.W, & Harrill A.H. (2014). Sensitivity to hepatotoxicity due to epigallocatechin gallate is affected by genetic background in diversity outbred mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 76, 19-26.
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