For hematoxylin and eosin (H&E) images, ATOs were embedded in Histogel (ThermoFisher Scientific, Grand Island, NY) and fixed overnight in 10% neutral-buffered formalin (ThermoFisher Scientific, Grand Island, NY). 5 μm sections and H&E staining were performed by the UCLA Translational Pathology Core Laboratory (TPCL). For immunofluorescence imaging, ATOs were isolated by cutting the culture insert around each ATO with a scalpel, followed by embedding the membrane and ATO in Tissue-Tek OCT (VWR Radnor, PA) and freezing on dry ice. 5 μm frozen sections were fixed in 10% neutral-buffered formalin and stained with anti-CD3 (clone UCHT1; Biolegend, San Diego, CA) at a 1:50 dilution overnight at 4°C followed by incubation with AlexaFluor 594-conjugated anti-mouse IgG (H+L) (Jackson ImmunoResearch, West Grove, PA) at room temperature. H&E and immunofluorescence images were acquired on a Zeiss AzioImager M2 with AxioCam MRM and AxioVision software (Zeiss, Jena, Germany).
Azioimager m2
Manufactured by Zeiss
The AzioImager M2 is a high-performance microscope imaging system designed for advanced scientific research and clinical applications. It features a modular and flexible design, allowing for a wide range of imaging techniques, including brightfield, phase contrast, and fluorescence microscopy. The system is equipped with a high-resolution digital camera and powerful image processing software, enabling detailed analysis and documentation of samples.
Automatically generated - may contain errors
Lab products found in correlation
Axiocam mrm, by Zeiss (2 mentions)
Tissue tek oct, by Avantor (2 mentions)
Neutral buffered formalin, by Thermo Fisher Scientific (2 mentions)
Histogel, by Thermo Fisher Scientific (2 mentions)
Axiovision, by Zeiss (2 mentions)
Anti cd3 clone ucht1, by BioLegend (2 mentions)
Alexafluor 594 conjugated anti mouse igg h l, by Jackson ImmunoResearch (2 mentions)
Neutral buffered formalin, by BioLegend (2 mentions)
2 protocols using azioimager m2
1
Histological and Immunofluorescent Analysis of Organoids
2
Histological and Immunofluorescent Analysis of Organoids
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For hematoxylin and eosin (H&E) images, ATOs were embedded in Histogel (ThermoFisher Scientific, Grand Island, NY) and fixed overnight in 10% neutral-buffered formalin (ThermoFisher Scientific, Grand Island, NY). 5 μm sections and H&E staining were performed by the UCLA Translational Pathology Core Laboratory (TPCL). For immunofluorescence imaging, ATOs were isolated by cutting the culture insert around each ATO with a scalpel, followed by embedding the membrane and ATO in Tissue-Tek OCT (VWR Radnor, PA) and freezing on dry ice. 5 μm frozen sections were fixed in 10% neutral-buffered formalin and stained with anti-CD3 (clone UCHT1; Biolegend, San Diego, CA) at a 1:50 dilution overnight at 4°C followed by incubation with AlexaFluor 594-conjugated anti-mouse IgG (H+L) (Jackson ImmunoResearch, West Grove, PA) at room temperature. H&E and immunofluorescence images were acquired on a Zeiss AzioImager M2 with AxioCam MRM and AxioVision software (Zeiss, Jena, Germany).
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