Commercial irregular and spherical powders, Al (Aluminum Industries Pvt Ltd., Hyderabad, India), Ti (CNPC Powder, Shanghai, China), Cu (SAFINA A.S., Vestec, Czech Republic), and Ti6Al4V (AP&C Powders Metallurgy, Montreal, Canada), respectively, were sprayed with Plasma Giken PCS100 (Saitana, Japan) equipment. The powder size distribution was analyzed with a laser diffraction particle sizing analyzer LS 13 320 Model Dry Powder System (Beckman Coulter, Brea, USA), while particle morphology was observed in a Phenom ProX Desktop scanning electron microscope (SEM) (Phenom-World BV, Eindhoven, The Netherlands).
Phenom prox desktop
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Phenom ProX Desktop is a compact, user-friendly scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of samples. It offers a simple and intuitive interface, automated functions, and advanced imaging capabilities.
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Lab products found in correlation
8 protocols using phenom prox desktop
1
Characterization of Metallic Powders
2
SEM Analysis of Impact Fracture Surfaces
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SEM micrographs of the
impact fractured sample break surfaces were obtained using a Phenom
ProX Desktop from Phenom-World BV (Eindhoven, Netherlands). Charging
was minimized by a Cressington 108 sputter coater (Watford, England)
to apply a thin gold coating on the fractured surface of the impact
bars, with a sputter duration of 10 s. The accelerating voltage of
the SEM was set to 10 kV, and the samples were examined at a magnification
of 1000×.
impact fractured sample break surfaces were obtained using a Phenom
ProX Desktop from Phenom-World BV (Eindhoven, Netherlands). Charging
was minimized by a Cressington 108 sputter coater (Watford, England)
to apply a thin gold coating on the fractured surface of the impact
bars, with a sputter duration of 10 s. The accelerating voltage of
the SEM was set to 10 kV, and the samples were examined at a magnification
of 1000×.
3
Ultrastructural Analysis of Extracellular Matrix
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After 14 days of culture, the cell loaded samples were fixed with 2.5% glutaraldehyde (Sigma Aldrich) in 0.1 M Sodium Cacodylate (Sigma Aldrich) buffer for 15 min and washed with 0.1 M Sodium Cacodylate buffer. Control samples without cells and cell loaded samples were dehydrated using 0.5 ml of multiple ethanol series (50% twice, 70% twice, 95% twice, 100% three times for 10 min) and were dried chemically with hexamethyldisilazane (Sigma Aldrich). Then the samples were washed three times with 0.5 ml ultrapure water for 5 min, dried by air and mounted on specimen stubs. To provide better contrast, only the samples for scanning electron microscopy (SEM) imaging were sputter coated with 8 nm gold (Q3150T, Quorum Technologies). SEM images were obtained using a Quanta 600 SEM (Thermo Scientific Breda, The Netherlands), in a high vacuum (<1.3 × 10−4) at 10 kV with a spot size of 3 using the Everhart‐Thornley secondary electron detector (ETD‐SE). Similar sample preparation was utilized to perform energy dispersive x‐ray (EDX) (Phenom ProX Desktop, ThermoFisher) analysis to evaluate regions in which extracellular matrix depositions were identified (10 kV, backscattered electron detector).
4
Scanning Electron Microscopy of Polyurethane Scaffolds
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A scanning electron microscope (Phenom ProX Desktop, ThermoFisher Scientific, Waltham, MA, USA) was employed to observe the porous structure of the polyurethane scaffolds. Cubic samples (2–3 mm per side) were analyzed. The cross sections of the samples were sputter coated with gold. SEM observation was carried out at 10 kV. The average pore size and analysis of pore size distribution in the samples were determined with ImageJ program. Pore sizes are expressed as mean +/− SD (n = 60).
5
Characterization of Multifunctional Materials
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1H-NMR spectra were obtained with a Bruker Avance II 400 MHz spectrometer (Milan, Italy).
FT-IR spectra were obtained with Bruker Alpha in the wave number range of 400 and 4000 cm−1 (Milan, Italy). SEM analyses were performed with Phenom Pro X Desktop (Thermo Fisher Scientific, Rome, Italy).
The rheological tests were carried out using a DHR-2 TA Instrument rotational rheometer (TA Instruments-Waters S.p.A., Sesto San Giovanni, Italy).
UV measurements were performed using an Eppendorf AF2200 spectrophotometer (Milan, Italy).
Cell cultures were performed using an Eppendorf New Brunswik S41i incubator (Milan, Italy).
Fluorescence images were obtained with an AxioVert200 (Zeiss) microscope (Milan, Italy).
A 3D printer Zmorph (Wroclaw, Poland) connected with a pressure controller OB1 working at pressures of 0–8000 mbar from Elveflow (Paris, France) was used to test printability of the inks.
FT-IR spectra were obtained with Bruker Alpha in the wave number range of 400 and 4000 cm−1 (Milan, Italy). SEM analyses were performed with Phenom Pro X Desktop (Thermo Fisher Scientific, Rome, Italy).
The rheological tests were carried out using a DHR-2 TA Instrument rotational rheometer (TA Instruments-Waters S.p.A., Sesto San Giovanni, Italy).
UV measurements were performed using an Eppendorf AF2200 spectrophotometer (Milan, Italy).
Cell cultures were performed using an Eppendorf New Brunswik S41i incubator (Milan, Italy).
Fluorescence images were obtained with an AxioVert200 (Zeiss) microscope (Milan, Italy).
A 3D printer Zmorph (Wroclaw, Poland) connected with a pressure controller OB1 working at pressures of 0–8000 mbar from Elveflow (Paris, France) was used to test printability of the inks.
6
Surface Morphology Analysis of PVC/SEBS Blends
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The analysis of surface morphology of neat PVC and SEBS and their blends loaded with the three different concentrations of IL was performed using a Thermo Phenom Prox desktop SEM with an integrated energy-dispersive X-ray (EDS) detector. Before SEM analysis, samples were placed in carbon tapes, dried, and sputter-coated with gold. Images were acquired at 15 KV and a working distance (WD) of 6.3–6.9 mm.
7
Surface Defects in Extruded Filaments
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A Phenom Pro X Desktop Scanning Electron Microscope (SEM) from ThermoFisher Scientific (Waltham, MA, USA) was used to investigate the surfaces of extruded filament samples at different screw frequencies. The images were acquired using a backscatter electron detector (BSD) technique with a beam energy of 15 keV with a magnification of 490:1.
The scope of the analysis was to investigate the presence of surface defects caused by prolonged residence times, or by the presence of solid particles in the metering section. The selected frequencies were 0.5, 5 and 20 rpm, based on model screening, allowing us to identify the position of the point of melt finalization. Two filament samples were always collected from the extrusion under steady state conditions, at a 15 min interval. The process was repeated using 2 nozzles with 0.8 and 1.5 mm final opening diameter, for a total of 12 samples.
The scope of the analysis was to investigate the presence of surface defects caused by prolonged residence times, or by the presence of solid particles in the metering section. The selected frequencies were 0.5, 5 and 20 rpm, based on model screening, allowing us to identify the position of the point of melt finalization. Two filament samples were always collected from the extrusion under steady state conditions, at a 15 min interval. The process was repeated using 2 nozzles with 0.8 and 1.5 mm final opening diameter, for a total of 12 samples.
8
Ultrastructural Analysis of ZIKV-Infected Monocytes
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ZIKV-infected monocytes cocultured with hCMEC/D3 cells on coverslips were washed twice with Cacodylate buffer (pre-warmed), and fixed with 2.5% glutaraldehyde for 1 h at RT. The samples were dehydrated in a graded alcohol series, coated with platinum, and imaged with a Phenom ProX Desktop scanning electron microscope (Thermo Fisher).
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