Drugs and antibodies were administered as described in the main text. Indomethacin, SC-236 and SC-560 were purchased from Sigma (St. Louis, MO), solvated in 1% Tween 80 in PBS, and 100 μL of each drug suspension or buffer alone per 20 mg body weight was administered by oral gavage. Clodronate and PBS liposomes were manufactured as previously described [32 (link), 33 (link)]. Rat anti-C-C chemokine receptor type 2 (CCR2) (clone MC-21) was generated as previously described [34 (link), 35 (link)]. Rat anti-lymphocyte antigen 6G (Ly6G) (clone 1AG) and rat IgG2a and IgG2b isotype controls were purchased from Bio X Cell (West Lebanon, NH). All antibodies were diluted in tissue culture grade sterile Dulbecco’s PBS and 200 μL of each antibody solution were injected intraperitoneally as indicated.
Rat anti lymphocyte antigen 6g ly6g clone 1ag
Manufactured by BioXCell
Rat anti-lymphocyte antigen 6G (Ly6G) (clone 1AG) is a monoclonal antibody that specifically recognizes the Ly6G cell surface marker. Ly6G is primarily expressed on neutrophils and can be used to identify and isolate this cell population.
Automatically generated - may contain errors
2 protocols using rat anti lymphocyte antigen 6g ly6g clone 1ag
1
Pharmacological Modulation of Inflammation
2
Pharmacological Modulation of Immune Responses
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Drugs and antibodies were administered as described in the main text. Indomethacin, SC-236 and SC-560 were purchased from Sigma (St. Louis, MO), solvated in 1% Tween 80 in PBS, and 100 μl of each drug suspension or buffer alone per 20 mg body weight was administered by oral gavage. Clodronate and PBS liposomes were manufactured as previously described (Seiler et al., 1997 (link), Zeisberger et al., 2006 (link)). Rat anti-C-C chemokine receptor type 2 (CCR2) (clone MC-21) was generated as previously described (Mack et al., 2001 (link), Bruhl et al., 2007 (link)). Rat anti-lymphocyte antigen 6G (Ly6G) (clone 1AG) and rat IgG2a and IgG2b isotype controls were purchased from Bio X Cell (West Lebanon, NH). All antibodies were diluted in tissue culture grade sterile Dulbecco's PBS and 200 μl of each antibody solution were injected intraperitoneally as indicated.
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