Total cell lysates or subcellular extracts were prepared and subjected to IB assays as described41 (link). The Santa Cruz anti-USP15 (2D5) and the Proteintech anti-USP15 antibodies were used for detecting human and mouse USP15, respectively. The Millipore anti-MDM2 and the Sigma anti-MDM2 antibodies were used for detecting human and mouse MDM2, respectively. For ubiquitination assays, MDM2 and NF-ATc2 were isolated by IP under denaturing conditions39 (link), and the ubiquitinated MDM2 and NFATc2 were detected by IB using a ubiquitin antibody specifically detecting K48-linked ubiquitin chains (Millipore). For transfection models, MDM2 or NFATc2 was transfected into HEK293 cells along with HA-ubiquitin, and the ubiquitinated MDM2 and NFATc2 were isolated by denaturing IP and detected by anti-HA IB. For deubiquitination assays, the isolated MDM2–ubiquitin conjugates were incubated with purified USP15 or its catalytic mutant in a deubiquitination buffer42 (link) for 4 h and then subjected to IB using anti-ubiquitin.
Ubiquitin antibody specifically
Manufactured by Merck Group
The Ubiquitin antibody is a laboratory reagent used to detect and quantify the presence of the ubiquitin protein in biological samples. Ubiquitin is a small, highly conserved protein that plays a crucial role in various cellular processes, including protein degradation, cell signaling, and protein trafficking. The Ubiquitin antibody is a tool used by researchers to study the function and regulation of ubiquitin in different biological systems.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ubiquitin antibody specifically
1
Investigating Ubiquitination and Deubiquitination of MDM2 and NFATc2
2
Investigating Ubiquitination and Deubiquitination of MDM2 and NFATc2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total cell lysates or subcellular extracts were prepared and subjected to IB assays as described41 (link). The Santa Cruz anti-USP15 (2D5) and the Proteintech anti-USP15 antibodies were used for detecting human and mouse USP15, respectively. The Millipore anti-MDM2 and the Sigma anti-MDM2 antibodies were used for detecting human and mouse MDM2, respectively. For ubiquitination assays, MDM2 and NF-ATc2 were isolated by IP under denaturing conditions39 (link), and the ubiquitinated MDM2 and NFATc2 were detected by IB using a ubiquitin antibody specifically detecting K48-linked ubiquitin chains (Millipore). For transfection models, MDM2 or NFATc2 was transfected into HEK293 cells along with HA-ubiquitin, and the ubiquitinated MDM2 and NFATc2 were isolated by denaturing IP and detected by anti-HA IB. For deubiquitination assays, the isolated MDM2–ubiquitin conjugates were incubated with purified USP15 or its catalytic mutant in a deubiquitination buffer42 (link) for 4 h and then subjected to IB using anti-ubiquitin.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!