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Pt3 ef1a bmi1 plasmid

Manufactured by Addgene
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The PT3-EF1a-BMI1 plasmid is a genetic construct that contains the BMI1 gene under the control of the EF1a promoter. The BMI1 gene is commonly used in research to study cellular processes and gene expression.

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Lab products found in correlation

Pgex 5x 1 expression vector, by GE Healthcare (2 mentions)

Close spelling variants of this product

PT3‐EF1a‐Bmi1

2 protocols using pt3 ef1a bmi1 plasmid

1

Purification and Binding Assay of GST-tagged Proteins

Cited 2 times
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GST, GST-MUC1-CD, and GST-MUC1-CD(AQA) were prepared as described (28 (link)). GST-tagged BMI1 was generated by PCR amplification of the pT3-EF1a-BMI1 plasmid (Addgene) and subcloning into the pGEX-5X-1 expression vector (GE Healthcare, Pittsburg, PA, USA). Purified GST-MUC1-CD and GST-MUC1-CD(AQA) were cleaved with thrombin to remove the GST moiety (30 (link)). For bindings assays, purified proteins were incubated for 2 h at room temperature. Adsorbates to glutathione-conjugated beads were analyzed by immunoblotting.
Hiraki M., Maeda T., Bouillez A., Alam M., Tagde A., Hinohara K., Suzuki Y., Markert T., Miyo M., Komura K., Ahmad R., Rajabi H, & Kufe D. (2016). MUC1-C ACTIVATES BMI1 IN HUMAN CANCER CELLS. Oncogene, 36(20), 2791-2801.
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2

Purification and Binding Assay of GST-tagged Proteins

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
GST, GST-MUC1-CD, and GST-MUC1-CD(AQA) were prepared as described (28 (link)). GST-tagged BMI1 was generated by PCR amplification of the pT3-EF1a-BMI1 plasmid (Addgene) and subcloning into the pGEX-5X-1 expression vector (GE Healthcare, Pittsburg, PA, USA). Purified GST-MUC1-CD and GST-MUC1-CD(AQA) were cleaved with thrombin to remove the GST moiety (30 (link)). For bindings assays, purified proteins were incubated for 2 h at room temperature. Adsorbates to glutathione-conjugated beads were analyzed by immunoblotting.
Hiraki M., Maeda T., Bouillez A., Alam M., Tagde A., Hinohara K., Suzuki Y., Markert T., Miyo M., Komura K., Ahmad R., Rajabi H, & Kufe D. (2016). MUC1-C ACTIVATES BMI1 IN HUMAN CANCER CELLS. Oncogene, 36(20), 2791-2801.
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