Avance ultrashield nmr spectrometer

Manufactured by Bruker

The Avance Ultrashield NMR spectrometer is a nuclear magnetic resonance spectrometer designed and manufactured by Bruker. It is capable of performing high-resolution NMR spectroscopy analysis on a variety of samples.

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Lab products found in correlation

Esquire 3000 spectrometer, by Bruker (2 mentions) Uv vis spectrometer, by PerkinElmer (1 mentions) Ct dna, by Merck Group (1 mentions) Cary eclipse fluorescence spectrometer, by Agilent Technologies (1 mentions)

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Avance spectrometer (419 citations) NMR spectrometer (293 citations) Avance NMR spectrometer (124 citations) Bruker spectrometers (71 citations) Ultrashield NMR spectrometer (8 citations) Avance Ultrashield spectrometer (5 citations) AVANCE 500) UltraShield-NMR spectrometer (3 citations) Avance 400 MHz Ultrashield NMR spectrometer (2 citations)

3 protocols using avance ultrashield nmr spectrometer

Synthesis and Characterization of HL-CH3(R)

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All chemicals were purchased from commercial sources and used as received, unless stated otherwise. ¹H and ¹³C NMR spectra were recorded using either a 400 or 500 MHz Bruker Avance Ultrashield NMR spectrometer at 296 K. Spectra were referenced internally by using the residual solvent (¹H δ = 3.34 and ¹³C δ = 49.86 for CD₃OD-d₄ relative to SiMe₄). ESI-MS spectra were recorded by Dr L. Haigh (Imperial College London) on a Bruker Daltronics Esquire 3000 spectrometer. HL-CH3(R) was synthesised and characterised according to published procedures.^{26 (link)}

Summers P.A., Thomas A.P., Kench T., Vannier J.B., Kuimova M.K, & Vilar R. (2021). Cationic helicenes as selective G4 DNA binders and optical probes for cellular imaging. Chemical Science, 12(43), 14624-14634.

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Characterization of Fluorescent Dyes and Oligonucleotides

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All chemicals were used as purchased from commercial sources unless stated otherwise. Solvents were distilled over an appropriate drying agent and degassed prior to use. ¹H and ¹³C NMR spectra were recorded by using a 400 or 500 mhz Bruker Avance Ultrashield NMR spectrometer at 296 K, respectively. Chemical shifts are referenced to residual deuterated solvent. Mass spectra were obtained by using electrospray ionisation (ESI) by Mrs. L. Haigh (Imperial College London) on a Bruker Daltronics Esquire 3000 spectrometer. Compound microanalysis was performed by Mr. A. Dickerson (Cambridge University). Absorption measurements were made on a Perkin–Elmer UV/Vis spectrometer. Emission spectra were obtained on a Varian Cary‐Eclipse fluorescence spectrometer. The oligonucleotides used were purchased RP‐cartridge purified from Eurogentec. CT‐DNA was obtained from Sigma–Aldrich. TOTA, TMPA‐M, ADOTA‐M,DAOTA‐M2,11 and 1,13‐dimethoxy‐5,9‐bis (propyl)‐5H‐quinolino[2,3,4‐kl]acridin‐9‐ium tetraflouroborate13 were prepared by previously reported procedures.

Shivalingam A., Vyšniauskas A., Albrecht T., White A.J., Kuimova M.K, & Vilar R. (2016). Trianguleniums as Optical Probes for G‐Quadruplexes: A Photophysical, Electrochemical, and Computational Study. Chemistry (Weinheim an Der Bergstrasse, Germany), 22(12), 4129-4139.

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Quantitative Analysis of Mannan Binding

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Supernatants were harvested from the alum/mannans mixture and were lyophilized. Lyophilized supernatants and a standard mannan sample (4.0 mg) that was not mixed with AH were respectively dissolved in 500 μl of deuterium oxide (99.9% D) with 0.01% (W/V) internal standard TMSP-2,2,3,3-D₄ (98.0% D). ¹H-NMR data were collected on a 400MHz Bruker Avance Ultra Shield NMR spectrometer at 295 K with the same acquisition parameters for all the samples. NMR spectra were processed using TOPSPIN 2.1 running on the Avance 400MHz NMR. The ring proton resonances (3.25 - 4.50 ppm) were integrated referencing to the integral of internal standard (−0.02 - 0.02 ppm) calibrated as 1.0. Based on the ratio between the mass of standard mannan (4.0 mg) and its ring proton integral (39.12), the mass of mannan in the supernatant was calculated using the detected ring proton integral multiplied by 4.0/39.2. Thus, the amount of mannan absorbed by the AH was determined by difference between the mass of total mannan added and the mass of mannan remaining in the supernatant after formulation.

Borriello F., Poli V., Shrock E., Spreafico R., Liu X., Pishesha N., Carpenet C., Chou J., Di Gioia M., McGrath M.E., Dillen C.A., Barrett N.A., Lacanfora L., Franco M.E., Marongiu L., Iwakura Y., Pucci F., Kruppa M.D., Ma Z., Lowman D.W., Ensley H.E., Nanishi E., Saito Y., O’Meara T.R., Seo H.S., Dhe-Paganon S., Dowling D.J., Frieman M., Elledge S.J., Levy O., Irvine D.J., Ploegh H.L., Wiliams D.L, & Zanoni I. (2022). An adjuvant strategy enabled by modulation of the physical properties of microbial ligands expands antigen immunogenicity. Cell, 185(4), 614-629.e21.

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