Jetprime in vitro dna

Retinal pigment epithelium (RPE) cell lines were used in this study and cultured as described in [22 (link)]. For the transfection of the plasmids or siRNA, jetPRIME in vitro DNA and an siRNA transfection reagent (PolyPlus Transfection, pt-114-15) were used following the manufacturer’s instructions. The siRNAs were ordered from RiboBio Company (Guangzhou, China) and are listed in Table 1.

Ishikawa cell line (Shanghai Huiying, Shanghai, China) and HEC-1A cell line (Genechem, Shanghai, China) were cultured in α-MEM medium (Bioind, Kibbutz Beit Haemek, Israel) and McCoy’s 5A medium (Bioind), respectively. The medium contained 10% fetal bovine serum (FBS) (Bioind) and 1% penicillin–streptomycin (Invitrogen). All cells were cultured in a humidified incubator at 37 °C with 5% CO₂. BTG1’s overexpression plasmid and knockdown plasmid were purchased from GeneChem (Shanghai, China), and both were transfected with jetPRIME^® in vitro DNA and siRNA Transfection Reagent (PolyPlus-transfection, France) for subsequent experiments. The related sequences can be found in Additional file 2: Table S2.