Mouse amh elisa kit

The concentration of serum AMH at the diestrus stage was determined using mouse AMH ELISA Kit (E-EL-M0113, Elabscience Biotechnology, Bethesda, MD) according to the manufacturer’s instructions. These samples were analyzed using a microplate reader to determine the amount of substrate converted at 450 nm.

Serum levels of AMH were measured using mouse AMH ELISA kit (Elabscience Biotechnology, Bethesda, MD, USA); serum FSH was measured using the FSH ELISA kit (Abnova, Taipei, Taiwan); and serum LH, testosterone, estradiol, and progesterone were measured using specific ELISA kits (Endocrine Technology, Inc, Newark, CA, USA). Inhibin B was measured using the mouse Inhibin B Enzyme Immunoassay Kit (RayBiotech, Norcross, GA, USA). All measurements were taken according to the manufacturer's instructions.

The six-week-old female mice were anesthetized, and blood was collected from the orbital vein behind the eyeball 14 days after chemotherapy. Sera were then separated by centrifugation (4000 × g, 15 min, 4°C) and frozen at -80°C. Serum AMH concentration was measured using a mouse AMH ELISA kit (Elabscience Biotechnology, Bethesda, MD, USA). The serum concentration of FSH was measured using a mouse follicle-stimulating hormone ELISA kit (Jianglai Biotechnology, Shanghai, China). The serum luteinizing hormone (LH) concentration was measured using a mouse luteinizing hormone ELISA kit (Jianglai Biotechnology, Shanghai, China). All measurements were performed in accordance with the manufacturer’s instructions.