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3 protocols using peroxidase conjugated anti flag m2

1

Antibody Characterization for Axon Guidance

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Scribble, Myc, and HA antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies for PlexinA3, PlexinA4, PlexinA2, PlexinD1, and NP-1 were from Santa Cruz Biotechnology, from Abcam (Cambridge, MA), from Cell Signaling Technology (Danvers, MA), and from R&D Systems (Minneapolis, MN). β-actin antibody was from Cell Signaling Technology. FLAG®M2, peroxidase-conjugated anti-FLAG®M2 and peroxidase-conjugated anti-HA antibodies were from Sigma (St. Louis, MO). Neuronal Class III β-Tubulin (Tuj1) antibody was from Covance (Princeton, NJ). Fluorescently conjugated Bovine Serum Albumin (BSA) was from Invitrogen Corporation (Carlsbad, CA). Analogue of cGMP (F-cGMP) - (8-[(2-[(fluoresceinylthioureido)amino]ethyl]thio] guanosine-3′,5′-cyclic monophosphate (8-Fluo-cGMP (F-cGMP) was from BIOLOG (Life Science Institute, Bremen, Germany). Recombinant Sema3A was kindly provided by T. Tran (Rutgers University, Newark, NJ).
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2

Antibody Characterization for Chromatin Study

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The following antibodies were used in this study: anti-HP1α (MBL; BMP001), anti-HP1β (1MOD-1A9; Millipore), anti-HP1γ (2MOD-1G6; Millipore), anti-DmHP1a (sc-26950; Santa Cruz), anti-Swi6 (41 (link)), peroxidase-conjugated anti-histone H3 (ab21054; Abcam), anti-CK2α (1AD9; Calbiochem), anti-α-tubulin (T5168; Sigma), peroxidase-conjugated anti-FLAG M2 (A8592; Sigma), peroxidase-conjugated anti-rabbit immunoglobulin G (IgG; A6667; Sigma), peroxidase-conjugated anti-mouse IgG (112–035- 072; Jackson ImmunoResearch) and peroxidase-conjugated anti-goat IgG (A5420; Sigma).
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3

Antibody Characterization for Axon Guidance

Check if the same lab product or an alternative is used in the 5 most similar protocols
Scribble, Myc, and HA antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies for PlexinA3, PlexinA4, PlexinA2, PlexinD1, and NP-1 were from Santa Cruz Biotechnology, from Abcam (Cambridge, MA), from Cell Signaling Technology (Danvers, MA), and from R&D Systems (Minneapolis, MN). β-actin antibody was from Cell Signaling Technology. FLAG®M2, peroxidase-conjugated anti-FLAG®M2 and peroxidase-conjugated anti-HA antibodies were from Sigma (St. Louis, MO). Neuronal Class III β-Tubulin (Tuj1) antibody was from Covance (Princeton, NJ). Fluorescently conjugated Bovine Serum Albumin (BSA) was from Invitrogen Corporation (Carlsbad, CA). Analogue of cGMP (F-cGMP) - (8-[(2-[(fluoresceinylthioureido)amino]ethyl]thio] guanosine-3′,5′-cyclic monophosphate (8-Fluo-cGMP (F-cGMP) was from BIOLOG (Life Science Institute, Bremen, Germany). Recombinant Sema3A was kindly provided by T. Tran (Rutgers University, Newark, NJ).
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