Cortisol kit 560360

The kits utilized for this project were from Cayman Chemicals (corticosterone: kit #16063; cortisol kit #560360) and the assays were run according to the manufacturer’s recommendations. Details of the kit verification have been reported previously (Tetel et al., 2022a (link); Tetel et al., 2022b (link)). Plates were incubated with samples overnight at 4°C. Plates were developed for 90 min and were read at 405 nm (SynergyLx, Biotek).

The kits utilized for this project were from Cayman Chemicals (corticosterone: kit #16063; cortisol kit #560360) and the assays were run according to the manufacturer’s recommendations. Details of the extensive kit verification have been reported previously (Tetel et al., 2022a (link); Tetel et al., 2022b (link)). Plates were incubated with samples overnight at 4°C. For the development of the plate, 250 determinations vial of Ellman’s Reagent was reconstituted with 50 mL of Ultrapure water. 200 μL of this reagent was added to each well on the plate before being placed on an orbital shaker for 90 min. At end of 90 min, plates were read at 405 nm (SynergyLx, Biotek).