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Cholic acid

Manufactured by Yuanye Bio-Technology
Sourced in China

Cholic acid is a primary bile acid produced by the liver. It is a key component in the bile, which is essential for the digestion and absorption of fats and fat-soluble vitamins. Cholic acid plays a crucial role in the emulsification and solubilization of lipids in the small intestine.

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2 protocols using cholic acid

1

Extraction and Analysis of Bile Acids

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Dulbecco’s Modified Eagle Medium (DMEM), Roswell Park Memorial Institute 1640 medium (RPMI 1640) and penicillin/streptomycin (PS) were purchased from Gibco (USA). The standards included lithocholic acid, ursodeoxycholic acid, chenodeoxycholic acid, cholic acid, deoxycholic acid, glycolithocholic acid, glycoursodeoxycholic acid, glycodeoxycholic acid, glycocholic acid, taurolithocholic acid, glycochenodeoxycholic acid, tauroursodeoxycholic acid, taurochenodeoxycholic acid, taurodeoxycholic acid, taurocholic acid were purchased from Yuanye Bio-Technology Co., Ltd (Shanghai, China). Estradiol was obtained from Sigma (San Francisco, US), and Estradiol-3-O-sulfate was ordered from Meilun Bio-Technology Co., Ltd (Dalian, China). Naringenin was purchased from Yuanye Bio-Technology Co., Ltd (Shanghai, China). The purity of standards was always more than 98%. Methanol and acetonitrile were derived from Merck (Darmstadt, Germany). The deionized water (18 MΩ) was from a MilliQ water purification system (Millipore, Billerica, MA). HLB solid phase extraction column was sourced from Waters (Milford, MA, USA).
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2

Phytochemical Characterization and Antioxidant Assays

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Folin-Ciocalteu phenol reagent, Na 2 CO 3 , formic acid, Al(NO 3 ) 3 , NaNO 2 , HCl, H 2 SO 4 , methanol, ethanol, and Trolox were obtained from Chengdu Kelong Chemical Reagent Works (Chengdu, China). 1,1-Diphenyl-2-picrylhydrazyl (DPPH, >99.7%), 2,2 ′ -azino-bis(3ethylbenzo thiazoline-6-sulfonic acid) diammonium salt (ABTS+, >99.7%), Oil red O (ORO), p-ntrophenyl laurate (pNP laurate), and chromatographic-grade methanol and acetonitrile were purchased from American Sigma (St. Louis, MO, USA). Standard compounds, including rutin, afzelin, astragalin, kaempferol-3-O-rutinoside, isoquercitrin, (+)-catechin, procyanidin B2, cyanidin-3-O-glucoside, 1,2,3,6-tetragalloylglucose, simvastatin, orlistat, glycocholic acid, cholic acid, taurocholic acid, porcine pancreatic lipase, and tris-HCl buffer solution, were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Standard compounds, including procyanidin B1, procyanidin B4, and procyanidin C1, were purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China).
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