Log In Sign up
The largest database of trusted experimental protocols

Diphteria toxin dt

Manufactured by Merck Group
Visit Supplier

Diphtheria toxin (DT) is a laboratory product used for research purposes. It is a protein that is produced by the bacterium Corynebacterium diphtheriae, the causative agent of diphtheria. DT is an essential tool for researchers studying the mechanisms of diphtheria and related diseases.

Automatically generated - may contain errors

Lab products found in correlation

Rat igg2b, by BioXCell (1 mentions) Anti cd8β, by BioXCell (1 mentions) Anti cd8α, by BioXCell (1 mentions) Rat igg1, by BioXCell (1 mentions)

Spelling variants of this product

Diphteria toxin (2 citations)

2 protocols using diphteria toxin dt

1

Depletion of Dendritic Cells In Vivo

Check if the same lab product or an alternative is used in the 5 most similar protocols
XCR1-DTRvenus (Yamazaki et al., 2013 (link)) and CD11c-DTR (Jung et al., 2002 (link)) mice were injected i.p. with diphteria toxin (DT, Sigma) or PBS as a control vehicle at a dose of 25 ng/g body weight (XCR1-DTRvenus) or 6 ng/g body weight (CD11c-DTR) every second day for a total of eight days. The efficiency of DC depletion was controlled by staining against CD11c and FACS analyses (data not shown).
Simmons S., Sasaki N., Umemoto E., Uchida Y., Fukuhara S., Kitazawa Y., Okudaira M., Inoue A., Tohya K., Aoi K., Aoki J., Mochizuki N., Matsuno K., Takeda K., Miyasaka M, & Ishii M. (2019). High-endothelial cell-derived S1P regulates dendritic cell localization and vascular integrity in the lymph node. eLife, 8, e41239.
+ Open protocol
+ Expand
2

Antibody-Mediated T Cell Depletion and Treg Abrogation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were given i.p. at 200µg per injection: anti-CD8α (Clone: 2.43; BioXcell), anti- CD8β (Clone: 53-5.8; BioXcell), anti-CD25 (Clone: PC61; eBioscience), as well as rat IgG2B (Clone: LTF-2; BioXcell) and rat IgG1 (Clone: HRPN; BioXcell) isotype controls. For in vivo TReg cell depletion with Diphteria Toxin (DT; Sigma-Aldrich), Foxp3DTR mice were given 50µg/kg of DT i.p. every other day as previously described (28 (link),29 (link)).
Rivas M.N., Lee Y., Wakita D., Chiba N., Dagvadorj J., Shimada K., Chen S., Fishbein M.C., Lehman T.J., Crother T.R, & Arditi M. (2017). CD8+ T cells contribute to the development of coronary arteritis in the Lactobacillus casei extract-induced murine model of Kawasaki Disease. Arthritis & rheumatology (Hoboken, N.J.), 69(2), 410-421.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!