Selexion tuning kit

Manufactured by AB Sciex

Sourced in Germany

The SelexION tuning kit is a specialized tool designed for the adjustment and optimization of the SelexION device, which is a differential ion mobility spectrometry (DIMS) system. The kit provides the necessary components and instructions to ensure the proper calibration and tuning of the SelexION system, enabling accurate and reliable performance for analytical applications.

Automatically generated - may contain errors

Lab products found in correlation

Robovials, by Thermo Fisher Scientific (4 mentions) Sciex lipidyzer platform, by AB Sciex (3 mentions) Lipidyzer internal standard mix, by AB Sciex (3 mentions) Omni bead ruptor elite, by Omni International (2 mentions) Methanol, by Avantor (2 mentions) 2 propanol, by Avantor (2 mentions) Ammonium acetate, by Avantor (2 mentions) System suitability test kits, by AB Sciex (2 mentions) Lipidyzer platform, by AB Sciex (1 mentions) 2.8 mm ceramic beads, by Omni International (1 mentions)

Spelling variants of this product

SelexIon (2 citations)

7 protocols using selexion tuning kit

Targeted Lipid Profiling from Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols

25 µl of plasma was pipetted into a glass tube for extraction. A modified Bligh and Dyer extraction (Bligh, 1959) is carried out on samples. Prior to biphasic extraction, a 13-lipid subclass Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers are dried down in a Genevac EZ-2 Elite. Lipid samples are resuspended in 1:1 methanol/dichloromethane with 10 mM Ammonium Acetate and transferred to robovials (Thermo 10800107) for analysis. Samples are analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of 1100 lipid species across 13 subclasses. Differential Mobility Device on Lipidyzer was tuned with SelexION tuning kit (Sciex 5040141). Instrument settings, tuning settings, and MRM list available upon request. Data analysis performed on Lipidyzer software. Quantitative values were normalized to volume. Samples were analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of lipids.

Middlekauff H.R., William K.J., Su B., Haptonstall K., Araujo J.A., Wu X., Kim J, & Sallam T. (2020). Changes in lipid composition associated with electronic cigarette use. Journal of Translational Medicine, 18, 379.

+ Open protocol

+ Expand

Targeted Lipidomic Analysis of Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lipids from 25 ul of plasma were extracted using a modified Bligh and Dyer extraction method by the UCLA lipidomics core facility⁵⁶. Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried down in a Genevac EZ-2 Elite evaporator. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10mM ammonium acetate and transferred to robovials (Thermo 10800107) for analysis. Samples were analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of 1100 lipid species across 13 classes. Differential Mobility Device on Lipidyzer was tuned with SelexION tuning kit (Sciex 5040141). Instrument settings, tuning settings, and MRM list are available upon request.

Fung T.C., Vuong H.E., Luna C.D., Pronovost G.N., Aleksandrova A.A., Riley N.G., Vavilina A., McGinn J., Rendon T., Forrest L.R, & Hsiao E.Y. (2019). Intestinal serotonin and fluoxetine exposure modulate bacterial colonization in the gut. Nature microbiology, 4(12), 2064-2073.

+ Open protocol

+ Expand

Quantitative Lipid Profiling of Liver

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 50–100 mg of frozen liver are homogenized in the Omni Bead Ruptor Elite with 2 mL homogenizer tube system (Omni, 19-628D). Samples are homogenized in cold phosphate-buffered saline (PBS) for 3 cycles of 10 s each at 5 m/s with a 10 s dwell time between cycles. A total of 3–6 mg of homogenized material are applied to a modified Bligh and Dyer extraction^{26 (link)}. Prior to biphasic extraction, a 13 lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers are dried down in a Genevac EZ-2 Elite. Lipid samples are resuspended in 1:1 methanol/dichloromethane with 10 mM ammonium acetate and transferred to robovials (Thermo 10800107) for analysis.
Samples are analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of 1100 lipid species across 13 lipid sub-classes. Differential Mobility Device on Lipidyzer is tuned with SelexION tuning kit (Sciex 5040141). Instrument settings, tuning settings, and MRM list available upon request. Data analysis performed on Lipidyzer software. Quantitative values are normalized to milligrams of material used.

Zhang Z., Feng A.C., Salisbury D., Liu X., Wu X., Kim J., Lapina I., Wang D., Lee B., Fraga J., Pan C., Williams K.J., Lusis A.J., Scumpia P, & Sallam T. (2020). Collaborative interactions of heterogenous ribonucleoproteins contribute to transcriptional regulation of sterol metabolism in mice. Nature Communications, 11, 984.

+ Open protocol

+ Expand

Targeted Lipidomic Analysis of Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Targeted Quantitative Lipidome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

50-100 mg of tissue were collected in tube pre-loaded with 2.8mm ceramic beads (Omni #19-628). Tissue is homogenized with PBS in the Omni Bead Ruptor Elite (3 cycles of 10 seconds at 5 m/s with a 10 second dwell time), and 3-6mg of homogenate were used for extraction (Bligh and Dyer extraction (Bligh, 1959)). Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried in a Genevac EZ-2 Elite. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10mM Ammonium Acetate and transferred to robovials (Thermo 10800107) for analysis. Samples were analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of 1100 lipid species across 13 classes. Differential Mobility Device on Lipidyzer is tuned with SelexION tuning kit (Sciex 5040141). Data analysis performed on Lipidyzer software and quantitative values were normalized to mg amounts used.

Palafox-Sánchez V., Ying Z., Royes L.F, & Gomez-Pinilla F. (2021). The Interaction between brain and liver regulates lipid metabolism in the TBI pathology. Biochimica et biophysica acta. Molecular basis of disease, 1867(4), 166078.

+ Open protocol

+ Expand

Quantitative Lipidomic Analysis of Human Plasma

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Methanol, 2-propanol, dichloromethane, water, and ammonium acetate were purchased from VWR International, LLC (Radnor, Radnor, PA, USA). All of these products were of HPLC grade. Internal standard (ISTD) kits (containing ISTDs for 13 lipid classes), pike standards with quality control plasma kits, SelexION tuning kits, and system suitability test kits for quantitative lipidomic analysis of human samples were purchased from AB Sciex Germany GmbH (Darmstadt, Germany). The composition of ISTD standard mixtures containing isotope-labeled lipid molecules was previously described in detail [64 (link),65 (link)]. Lipids were extracted from the plasma samples using a modified Bligh-Dyer method [66 (link)].

Szász I., Koroknai V., Várvölgyi T., Pál L., Szűcs S., Pikó P., Emri G., Janka E., Szabó I.L., Ádány R, & Balázs M. (2024). Identification of Plasma Lipid Alterations Associated with Melanoma Metastasis. International Journal of Molecular Sciences, 25(8), 4251.

+ Open protocol

+ Expand

Quantitative Lipidomics Analysis Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Methanol, 2-propanol, dichloromethane, water, ammonium-acetate were purchased from VWR International, LLC (Radnor, Radnor, PA, USA). All of them were of HPLC grade. Internal standard (ISTD) kits for quantitative lipidomic analysis of human samples were bought from AB Sciex Germany GmbH (Darmstadt, Germany). The kits contain ISTDs for 13 lipid classes including ceramides (Cers), cholesterolesters (CEs), DGs, acylceradmides (ACer), fatty acids (FAs), hexosylceramides (HexCers), lactosylceramides (LacCers), LPCs, LPEs, PCs, phosphatidylethanolamines (PEs), SMs, and TGs. The composition of ISTD standard mixtures containing isotope-labelled lipid molecules were described in detail previously [18 (link)]. Spike standards with quality control plasma kits, SelexION tuning kits, and system suitability test kits were also obtained from AB Sciex Germany GmbH.

Pikó P., Pál L., Szűcs S., Kósa Z., Sándor J, & Ádány R. (2021). Obesity-Related Changes in Human Plasma Lipidome Determined by the Lipidyzer Platform. Biomolecules, 11(2), 326.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!