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5 protocols using disodium phosphate

1

Horseradish Peroxidase Conjugation Protocol

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Potassium permanganate, barium chloride, hydrochloric acid, chloroacetic acid, ethanol, ammonium hydroxide aqueous solution (25%, by mass), iron(II) sulfate heptahydrate, hydrogen peroxide, sodium dihydrogen phosphate, disodium phosphate, glutaraldehyde, sulfuric acid, phenol, 4-aminoantipyrine (4-AAP) and sodium hydroxide were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, PR China). Iron(III) chloride hexahydrate, polyethylene glycol (PEG), graphite and sodium nitrate were bought from Aladdin Industrial Corporation (Shanghai, PR China). The 6arm-PEG-NH2 was supplied by Shanghai Jinpan Biotech Co., Ltd. (Shanghai, PR China). Horseradish peroxidase (HRP) was bought from Shanghai Jinsui Biotechnology Co., Ltd (Shanghai, PR China). The 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDAC) was bought from Sangon Biotech Co., Ltd (Shanghai, PR China). All other reagents were of analytical grade and used without any further treatment. Deionized water was used in all the experiments.
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2

Comprehensive Analytical Reagents Protocol

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Tetrabutyl orthotitanate (TBOT), boric acid, ammonium chloride, disodium phosphate, sodium dihydrogen phosphate, methanol, ethanol, acetonitrile, 3,3′,5,5′-tetramethyl benzidine, glucose, fructose, maltose, galactose, lactose, citric acid were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Uric acid, lauric acid, ascorbic acid, L-aspartic acid were purchased from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). Bovine serum albumin (BSA), Human serum albumin (HSA), horse radish peroxidase, glucose oxidase, L-cysteine, glutathione, L-phenylalanine, glycine, L-tryptophan, L-methionine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Human serum was purchased from Solarbio (Beijing, China).
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3

Antioxidant Evaluation of Bioactive Compounds

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Isoeugenol, shikonin, baicalein, rosmarinic acid, dihydromyricetin, l-3,4-dihydroxyphenylalanine (l-DOPA), tyrosinase (EC 1.14.18.1), phenanthroline, pyrogallol, 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), diphenyl-2-picrylhydrazyl (DPPH), thiobarbituric acid (TBA), and 2,2′-azobis(2-methylpropionamidine)dihydrochloride (AAPH) were purchased from the Sigma Chemical Company (St. Louis, MO, USA). C3606 reagent kit for organization mitochondria separation was purchased from Shanghai Biyuntian company. Disodium phosphate, sodium dihydrogen phosphate, K2S2O8, potassium sulfate, and ferrous sulfate were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). All other solvents and chemicals with analytical grade were commercially available. The Minimum Standards of Reporting Checklist contains details of the experimental design, and statistics, and resources used in this study (Additional file 1).
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4

Physicochemical Characterization of Oxyresveratrol

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HP-β-CD and β-CD were purchased from Jiangsu Fengyuan Biotechnology Co., Ltd. (Suqian, China). Oxyresveratrol was purchased from Hangzhou Great Forest Biomedical Ltd. (Hangzhou, China). Sodium dihydrogen phosphate and disodium phosphate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). HPLC grade methanol were purchased from J&K Scientific (Beijing, China). HPLC grade formic acid was purchased from Shanghai Aladdin Chemical Reagent Co., Ltd. (Shanghai, China).
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5

Tetracycline antibiotics evaluation

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Tetracycline, chlorTetracycline, oxyTetracycline, and doxycycline were purchased from Meilun Biotechnology Co., Ltd. (Dalian, China). Micrococcus CAT was purchased from Sigma (Saint-Quentin Fallavier, France). Hydrochloric acid, sulfuric acid, sodium hydroxide, H2O2, sodium dihydrogen phosphate, and disodium phosphate were purchased from Sinopharm (Shanghai, China). Methanol and ethanol were obtained from Fisher Chemicals (Fair Lawn, NJ, USA).
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