Cells were harvested using trypsin/EDTA solution to obtain a single cell suspension. The cells were immunostained in 1% horse serum (Gibco) in sterile phosphate buffered saline (PBS) with primary antibodies conjugated with fluorescent dye. The antibodies were PerCP-conjugated anti-CD45 (Immuno Tools, Friesoythe, Germany), FITC conjugated anti-human CD44 (BD Bioscience Pharmingen, NJ, USA), PE-conjugated anti-human CD105 (Immuno Tools), and APC-conjugated anti-human CD90 (Immuno Tools). Flow cytometry analysis was performed using a FACSCalibur Flow cytometer (BD Bioscience, CA, USA).
Pe conjugated anti human cd105
Manufactured by Immunotools
Sourced in United States, Germany
PE-conjugated anti-human CD105 is a laboratory reagent used for the identification and analysis of cells expressing the CD105 (endoglin) antigen. CD105 is a transmembrane glycoprotein that plays a role in angiogenesis and is expressed on endothelial cells, activated monocytes, and certain types of cancer cells. The PE (phycoerythrin) fluorescent label allows for the detection and quantification of CD105-positive cells using flow cytometry and other analytical techniques.
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Lab products found in correlation
Percp conjugated anti cd45, by Immunotools (2 mentions)
Apc conjugated anti human cd90, by Immunotools (2 mentions)
Fitc conjugated anti human cd44, by BD (2 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Fascalibur, by BD (1 mentions)
Cellquest software, by BD (1 mentions)
2 protocols using pe conjugated anti human cd105
1
Multiparametric Flow Cytometry Analysis
2
Immunophenotyping of Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were detached with trypsin/EDTA solution to obtain single-cell suspension. Further, cells were washed with 1% FBS in PBS and subsequently stained with antibodies. Primary antibodies were FITC conjugated anti-human CD44 (BD Bioscience Pharmingen, USA), APC-conjugated anti-human CD90 (Immuno Tools, Germany), PE-conjugated anti-human CD105 (Immuno Tools) and PerCP-conjugated anti-CD45 (Immuno Tools). Stained cells were analysed using a FASCalibur using the CellQuest software (BD Bioscience, USA).
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