Angelicin

Manufactured by Merck Group

Sourced in United States, Germany

Angelicin is a laboratory reagent used as a photosensitizer in various scientific and research applications. It exhibits the ability to absorb light energy and transfer it to other molecules, triggering specific reactions. The core function of Angelicin is to facilitate photochemical processes in experimental settings.

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3 protocols using angelicin

Extraction and Characterization of Bioactive Compounds from Myrica gale

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Myrigalone A, myrigalone B and myrigalone D, and 2′,4′-dihydroxy-6′-methoxy-3′5′-dimethylchalcone (DMC) were extracted from Myrica gale fruits and plants as described [36 (link)] by Syngenta’s Jealott’s Hill International Research Centre (Bracknell, UK). Gibberellin A₄₊₇ and paclobutrazol were purchased from Duchefa Biochemie (Haarlem, The Netherlands). Phloretin, dihydrochalcone, naringenin, neohesperidin dihydrochalcone, daphnetin, psoralen, angelicin, ferulic acid, acacetin, carbonyl cyanide m-chlorophenyl hydrazone (CCCP), 2,3,5-triiodobenzoic acid (TIBA), indole-3-acetic acid (IAA), aminoethoxyvinylglycine (AVG), L-kynurenine, and hydrogen peroxide solution (30% (w/w)) were purchased from Sigma-Aldrich (St Louis, MO, USA). 5-(4-Chlorophenyl)-4H-1,2,4-triazole-3-thiol (yucasin) was purchased from Carbosynth Ltd. (Compton, Berkshire, UK). 4-(2,4-dimethylphenyl)-2-(1H-indol-3-yl)-4-oxobutanoic acid (auxinole) was purchased from Cambridge Bioscience (Cambridge, UK). All the compounds were dissolved in DMSO except for Phloretin, dihydrochalcone, naringenin and neohesperidin dihydrochalcone which were dissolved in methanol. Controls were performed with basal solvent (0.1% (v/v) DMSO or methanol) as appropriate.

Nakabayashi K., Walker M., Irwin D., Cohn J., Guida-English S.M., Garcia L., Pavlović I., Novák O., Tarkowská D., Strnad M., Pérez M., Seville A., Stock D, & Leubner-Metzger G. (2022). The Phytotoxin Myrigalone A Triggers a Phased Detoxification Programme and Inhibits Lepidium sativum Seed Germination via Multiple Mechanisms including Interference with Auxin Homeostasis. International Journal of Molecular Sciences, 23(9), 4618.

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Angelicin's Anti-Cancer Mechanism

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Angelicin, dimethyl sulfoxide (DMSO), DAPI, Cell Counting kit 8 (CCK8) assay kit (06432379001), LY294002 hydrochloride (L9908), and 5-bromo-2-deoxyuridine (BrdU) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Polyclonal antibodies against PI3K (AF3242), Akt (AF6261), phosphorylated (p)-Akt (AF0016), apoptosis regulator BAX (Bax; AF0083) and Bcl-2 (AF6139) were purchased from Affinity Biologicals, Inc. (Ancaster, ON, Canada); antibodies against caspase-9 (9508T), caspase-3 (9665S), cytochrome c (4272S), Ki-67 (12075) and p-vascular endothelial growth factor receptor (VEGFR) 2 (9698) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Horseradish peroxidase-conjugated anti-GAPDH antibody (10494–1-AP), goat anti-mouse immunoglobulin (Ig) G (10317–1-AP) and goat anti-rabbit IgG (10285–1-AP), were obtained from ProteinTech Group, Inc. (Chicago, IL, USA). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin and streptomycin were purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA). A protease inhibitor cocktail was obtained from Roche Diagnostics (Basel, Switzerland). The bicinchoninic acid (BCA) protein assay and the enhanced chemiluminescence (ECL) kits were obtained from Pierce (Thermo Fisher Scientific, Inc.).

Wang F., Li J., Li R., Pan G., Bai M, & Huang Q. (2017). Angelicin inhibits liver cancer growth in vitro and in vivo. Molecular Medicine Reports, 16(4), 5441-5449.

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Ubiquitin Topoisomers Biochemical Characterization

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Ubiquitin monomer, BSA, Tris, Triton X-100 and DTT, trimethyl-psoralen (TMP), hydroxyurea, angelicin and BrdU were purchased from Sigma-Aldrich. Di-ubiquitin topoisomers (linear, K6-, K11-, K27-, K29-, K33-, K48- and K63-linked ubiquitin–ubiquitin) were purchased from Boston Biochem. Complete protease inhibitors cocktail tablets were obtained from Roche. Tween-20, Colloidal blue staining kit and precast SDS–polyacrylamide Bis-Tris gels (Invitrogen). MMC was from Duchefa.
All recombinant proteins, plasmids and antibodies generated for the present study are available upon request and described in further detail on our reagents website (https://mrcppureagents.dundee.ac.uk/).

Perez-Oliva A.B., Lachaud C., Szyniarowski P., Muñoz I., Macartney T., Hickson I., Rouse J, & Alessi D.R. (2014). USP45 deubiquitylase controls ERCC1–XPF endonuclease-mediated DNA damage responses. The EMBO Journal, 34(3), 326-343.

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