α methylenecyclopropyl glycine mcprg

(S)-hypoglycin A (HGA, purity 85%) and α-(methylenecyclopropyl)glycine (MCPrG) standards were purchased from Toronto Research Chemicals (Toronto, ON, Canada). Acetonitrile (ACN), methanol (MeOH), formic acid (FA) and ammonium formate (NH₄COOH) were purchased from Merck (Darmstadt, Germany). Double-deionized water was obtained using a Milli-Q system from Merck (Merck Millipore, Darmstadt, Germany).

(S)-hypoglycin A (HGA, purity 85%), α-(methylenecyclopropyl)glycine (MCPrG, 97%), and MCPA-carnitine (97%) standards were purchased from Toronto Research Chemicals (Toronto, Canada). MCPA-glycine (97%) and MCPF-glycine (97%) standards were purchased from IsoSciences (Ambler, PA, USA). Although the purity of HGA standard (85%) is relatively low, the analysis showed that it does not contain any of the related analytes. Acetonitrile (ACN), methanol (MeOH), ethylenediaminetetraacetic acid (EDTA), formic acid (FA), and ammonium formate (NH₄COOH) were of LC–MS grade and purchased from Merck (Darmstadt, Germany). C-₁₈ material (Polygoprep 300-30C₁₈) was purchased from Macherey–Nagel (Düren, Germany). Double-deionized water was obtained using a Milli-Q system from Merck (Merck Millipore, Darmstadt, Germany).