3h glutamine

Manufactured by PerkinElmer

[3H]-glutamine is a radiolabeled amino acid used in various research applications. It serves as a precursor for the synthesis of proteins and other biomolecules, allowing for the investigation of metabolic pathways and cellular processes.

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Lab products found in correlation

3h tryptophan, by PerkinElmer (2 mentions) 14c lactate, by PerkinElmer (1 mentions) 14c citrate, by Moravek Biochemicals (1 mentions) 3h serine, by PerkinElmer (1 mentions) 3h glutamic acid, by PerkinElmer (1 mentions) 3h proline, by PerkinElmer (1 mentions) 3h glycine, by PerkinElmer (1 mentions) 3h arginine, by PerkinElmer (1 mentions) 3h tyrosine, by PerkinElmer (1 mentions) 3h phenylalanine, by PerkinElmer (1 mentions) 3h leucine, by PerkinElmer (1 mentions)

Close spelling variants of this product

[3H]-L-glutamine (20 citations) L-[3,4-3H(N)]Glutamine (4 citations) L-2,3,4-[3H]glutamine (2 citations) 3H-labeled glutamine (2 citations)

3 protocols using 3h glutamine

Synthesis of Novel Sulfonylpiperidine Compounds

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BI01383298, 1-(3,5-Dichlorophenylsulfonyl)-N-(4-fluorobenzyl)piperidine-4-carboxamide, was purchased from Medkoo Biosciences (Chapel Hill, NC, U.S.A.). BI01372674, chemically analogous to BI01383298, was provided by Boehringer Ingelheim Pharma GmbH (Ingelheim, Germany). PF06761281, (2R)-2-Hydroxy-2-[2-(2-methoxy-5-methyl-3-pyridinyl)ethyl]succinic acid, was kindly supplied by Pfizer Inc. (New York, NY, U.S.A.). [¹⁴C]-Citrate (specific radioactivity, 113 mCi/mmol) and [¹⁴C]-nicotinate (specific radioactivity, 50 mCi/mmol) were purchased from Moravek Biochemicals (Brea, CA, U.S.A.). [³H]-Arginine monohydrochloride (specific radioactivity, 54.5 mCi/mmol), [¹⁴C]-lactate (specific radioactivity, 154.8 mCi/mmol), [³H]-glutamine (specific radioactivity, 50.5 mCi/mmol), and [³H]-tryptophan (specific radioactivity, 20.1 mCi/mmol) were purchased from PerkinElmer (Waltham, MA, U.S.A.). All other reagents were from Millipore-Sigma (St. Louis, MO, U.S.A.) or Thermo Fisher Scientific (Waltham, MA, U.S.A.).

Higuchi K., Kopel J.J., Sivaprakasam S., Jaramillo-Martinez V., Sutton R.B., Urbatsch I.L, & Ganapathy V. (2020). Functional analysis of a species-specific inhibitor selective for human Na+-coupled citrate transporter (NaCT/SLC13A5/mINDY). Biochemical Journal, 477(21), 4149-4165.

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Glutamine Uptake in Pancreatic Cancer

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Pancreatic cancer cells were seeded in 24 well plates. At the end of the treatment, the control wells were treated with 4 mM of glutamine for 10 min, followed by addition of 4 μl of [³H]-glutamine (Perkin Elmer). The cells were washed and lysed in 1% SDS followed by measurement of radiolabel using a scintillation counter. The raw values were normalized to cell counts.

Shukla S.K., Purohit V., Mehla K., Gunda V., Chaika N.V., Vernucci E., King R.J., Abrego J., Goode G.D., Dasgupta A., Illies A.L., Gebregiworgis T., Dai B., Augustine J.J., Murthy D., Attri K.S., Mashadova O., Grandgenett P.M., Powers R., Ly Q.P., Lazenby A.J., Grem J.L., Yu F., Matés J.M., Asara J.M., Kim J.W., Hankins J.H., Weekes C., Hollingsworth M.A., Sarkova N.J., Sasson A.R., Fleming J.B., Oliveto J.M., Lyssiotis C.A., Cantley L.C., Berim L, & Singh P.K. (2017). MUC1 and HIF-1alpha Signaling Crosstalk Induces Anabolic Glucose Metabolism to Impart Gemcitabine Resistance to Pancreatic Cancer. Cancer cell, 32(1), 71-87.e7.

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Radiolabeled Amino Acids for Protein Synthesis

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³H-Leucine (leucine, l-[4,5-³H]), ³H-phenylalanine (phenylalanine, l-[2,3,4,5,6-³H]), ³H-tryptophan (tryptophan, l-[5-3H(N)]), ³H-methionine (l-[methyl-³H]-methionine), ³H-tyrosine (l-[ring-3,5-³H]-tyrosine), ³H-arginine (arginine monohydrochloride l-[2,3,4-³H]-arginine), ³H-lysine (l-[4,5-³H(N)]-lysine), ³H-proline (l-[2,3,4,5-³H]-proline), ³H-serine (l-[³H(G)]-serine), ³H-glycine (glycine, [2-³H]-glycine), ³H-glutamine (l-[3,4-³H(N)]-glutamine), and ³H-glutamic acid (l-[3,4-³H]-glutamic acid) are from PerkinElmer (Waltham, MA).

Bandyopadhyay U., Todorova P., Pavlova N.N., Tada Y., Thompson C.B., Finley L.W, & Overholtzer M. (2022). Leucine retention in lysosomes is regulated by starvation. Proceedings of the National Academy of Sciences of the United States of America, 119(6), e2114912119.

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