Small interfering RNA (siRNA) oligonucleotides targeting SPSB2 and control nonspecific siRNA were purchased from Santa Cruz Biotechnology. In twelve-well plates, cells were seeded the day before transfection and grown to 30% confluence. siRNA oligonucleotides (100 nM) were transfected into cells by using Lipofectamine 2000 reagents. After 48 h of transfection, cells were subjected to further experiments.
Control nonspecific sirna
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Control nonspecific siRNA is a laboratory reagent used to monitor the effects of small interfering RNA (siRNA) transfection. It is a non-targeting siRNA that does not recognize any known mammalian gene, allowing researchers to assess the specificity of their siRNA experiments.
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Lab products found in correlation
3 protocols using control nonspecific sirna
1
Silencing SPSB2 Gene Expression
2
siRNA Targeting of PPARα Modulation
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Double strand interfering RNA (siRNA) targeting human PPARα and a control non-specific siRNA (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were transfected by means of the Lipofectamine RNAiMAX Reagent (Invitrogen, San Diego, CA). Twenty-four h after siRNA administration, cells were treated with aspirin (50 μm ) or PPARα agonist (WY14643) (1 μm ) (SIGMA Chemicals Company, St Louis, MO, USA). Forty-eight h after treatment, cells were processed for RNA and protein analysis.
3
Silencing HSF1 in Cell Lines
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The siRNA oligonucleotides targeting HSF1 and control nonspecific siRNA were purchased from Santa Cruz Biotechnology. In 6-well plates, cells were plated the day before transfection and grown to 30–50 % confluence. The siRNA oligonucleotides (100 nM) were transfected into cells by using Lipofectamine 2000 reagents (Invitrogen). After 48 h of transfection, cells were subjected to further treatments and analysis.
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