Microscopic observations were performed using a fluorescence microscope (BX-61, Olympus, Tokyo, Japan) with the following filter sets: blue (450−490 nm, exciting filter BP 490), green (545 nm, exciting filter BP 545). Images were obtained with an Olympus CCD DP70 digital camera. Fluorescence intensity analysis (DHFDA and JC-1 expression) was performed using ImageJ version 1.52a (NIH, Bethesda, MD, USA). Data were normalized with respect to non-irradiated control values.
Ccd dp70
Manufactured by Olympus
Sourced in United States
The CCD DP70 is a digital color camera designed for microscopy imaging. It features a high-resolution CCD sensor that captures detailed images of samples under a microscope. The camera is capable of capturing still images and video footage, and its output can be connected to a computer for further analysis and processing.
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Lab products found in correlation
5 protocols using ccd dp70
1
Fluorescence Microscopy Analysis of Cellular Markers
2
Fluorescence Microscopy Imaging Analysis
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Microscopic observations were performed using a fluorescence microscope (BX-61, Olympus, Tokyo, Japan) with the following filter sets: blue (450–490 nm, exciting filter BP 490), green (545 nm, exciting filter BP 545). Images were obtained with an Olympus CCD DP70 digital camera. Fluorescence intensity analysis was performed using ImageJ version 1.52a (NIH, Bethesda, MD, USA). Data were normalized with respect to non-irradiated control values.
3
Epifluorescence Microscopy for Imaging
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Microscopic observations were carried out using an Olympus BX61 epifluorescence microscope equipped with filter sets for fluorescence microscopy: ultraviolet (exciting filter BP360–390), blue (exciting filter BP460–490), and green (exciting filter BP510–550). Photographs were obtained with the digital camera Olympus CCD DP70 and processed using the Adobe Photoshop CS5 extended version 12.0 software (Adobe Systems Inc., San Jose, CA, USA).
4
Epifluorescence Microscopy for Imaging
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Microscopic observations were carried out using an Olympus BX61 epifluorescence microscope equipped with filter sets for fluorescence microscopy: ultraviolet (exciting filter BP360-390), blue (exciting filter BP460-490), and green (exciting filter BP510-550). Photographs were obtained with the digital camera Olympus CCD DP70 and processed using the Adobe Photoshop CS5 extended version 12.0 software (Adobe Systems Inc., San Jose, CA, USA).
5
Microscopic Epifluorescence Imaging Techniques
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Microscopic observations were carried out using an Olympus BX61 epifluorescence microscope equipped with green filter (exciting filter BP510-550). Photographs were obtained with the digital camera Olympus CCD DP70 and processed using the Adobe Photoshop CS5 extended version 12.0 software (Adobe Systems Inc., San Jose, CA, USA).
Data are expressed as the mean value of at least three experiments ± standard deviations (SD). The statistical significance was determined using analysis of variance (ANOVA) followed by Bonferroni’s test, and p < 0.05 was considered statistically significant.
Data are expressed as the mean value of at least three experiments ± standard deviations (SD). The statistical significance was determined using analysis of variance (ANOVA) followed by Bonferroni’s test, and p < 0.05 was considered statistically significant.
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