Cd14 cells

Manufactured by STEMCELL

CD14+ cells are a specific type of immune cells that play a key role in the body's defense against infections and inflammation. They are a subset of monocytes, which are a type of white blood cell. CD14+ cells express the CD14 surface marker, which allows them to recognize and respond to bacterial components. These cells are important for innate immunity and can phagocytose and kill pathogens.

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Lab products found in correlation

Mpla sm, by InvivoGen (1 mentions) E coli lps, by Merck Group (1 mentions) Human fc block, by BD (1 mentions) Interleukin 4 (il 4), by BD (1 mentions) Anti tigit clone mbsa43, by Thermo Fisher Scientific (1 mentions) Anti pd 1 clone j116, by Thermo Fisher Scientific (1 mentions) Anti tim 3 clone f382e2, by BioLegend (1 mentions) Gm csf, by Miltenyi Biotec (1 mentions) Easysep magnet, by STEMCELL (1 mentions) Rnalater, by Merck Group (1 mentions) Easysep human cd14 positive selection kit 2, by STEMCELL (1 mentions)

Close spelling variants of this product

CD14+ cell positive selection (3 citations)

3 protocols using cd14 cells

Generating Monocyte-Derived Dendritic Cells

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To obtain human moDCs, blood samples were obtained from advanced stage PaC patients after providing written informed consent (Dr. George Zogopolous, McGill University Health Centre). Patient moDCs were generated from peripheral blood monocytes isolated by positive selection of CD14⁺ cells (StemCell Technologies, Inc., cat# 18058). Human monocytes were cultured in monocyte attachment media for 1 h. After 1 h, the media was replaced with DC differentiation (StemXVivo serum-free DC base media, cat# CCM003) supplemented with GM-CSF (50 ng/mL, cat# 300–03) and IL-4 (35 ng/mL, cat# 200–04) and 1B/TC inh. Monocytes were cultured for 6 d in the differentiation media. On day 3, half the volume of the differentiation media was replaced with new media and cytokines. On the day 6, the media was replaced with maturation media containing monophosphoryl lipid A (MPLA-SM, 2 μg/mL, InvivoGen, cat# vac-mpla),³³ (link) IFNγ (1000 U/mL, PeproTech, cat# 315–05) and 1B/TC inhibitor (4 μM).

Penafuerte C., Feldhammer M., Mills J.R., Vinette V., Pike K.A., Hall A., Migon E., Karsenty G., Pelletier J., Zogopoulos G, & Tremblay M.L. (2017). Downregulation of PTP1B and TC-PTP phosphatases potentiate dendritic cell-based immunotherapy through IL-12/IFNγ signaling. Oncoimmunology, 6(6), e1321185.

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Monocyte-derived Dendritic Cell Activation

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Monocyte derived dendritic cells (DCs) were produced after selection of CD14+ cells (Stem Cell Technologies) from PBMCs isolated from a Buffy Coat, using the manufacturer’s instructions. CD14+ monocytes were cultured for 5 days in 7.5 ng/ml IL-4 (BD) and 20 ng/mL GMC-SF (Miltenyi Biotec). On day 4, 1 ng/ml of E. coli LPS (Sigma) was added to the cultures to mature the dendritic cells. Previously frozen PBMCs from patients and healthy controls were thawed and co-cultured with allogeneic DCs at 1:10 DCs to PBMCs with 5 μg/ml of blocking antibodies: anti-PD-1 (Clone J116, eBioScience), anti-TIGIT (Clone MBSA43, eBioScience), anti-TIM-3 (Clone F38-2E2, BioLegend), or Isotype control (Clone P3.6.2.8.1, eBioScience) in the presence of human Fc Block (BD). Cultures were kept at 37°C and 5% CO₂ for five days and supernatants were collected and stored at −80°C.

Fleury M., Belkina A.C., Proctor E.A., Zammitti C., Simms R.W., Lauffenburger D.A., Snyder-Cappione J.E., Lafyatis R, & Dooms H. (2018). INCREASED EXPRESSION AND MODULATED REGULATORY ACTIVITY OF CO-INHIBITORY RECEPTORS PD-1, TIGIT AND TIM-3 IN LYMPHOCYTES OF SYSTEMIC SCLEROSIS PATIENTS. Arthritis & rheumatology (Hoboken, N.J.), 70(4), 566-577.

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Magnetic Isolation of Human Monocytes

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Monocytes were isolated from PBMC by incubation with anti-CD14 antibody coupled to magnetic microspheres (EasySep Human CD14 Positive Selection Kit II), the tube with the mixture was placed on an EasySep magnet for immunomagnetic positive selection of CD14 + cells (StemCell). The purity of the magnetic selection was ≥95% in all cases. Monocytes were used for cell culture and an aliquot stored in RNAlater (Sigma Aldrich) for RNA isolation.

Berenice Martínez-Shio E., Martín Cárdenas-Hernández Á., Jiménez-Suárez V., Sherell Marín-Jáuregui L., Castillo-Martin Del Campo C., González-Amaro R., Escobedo-Uribe C.D, & Monsiváis-Urenda A.E. (2022). Differentiation of circulating monocytes into macrophages with metabolically activated phenotype regulates inflammation in dyslipidemia patients. Clinical and experimental immunology, 208(1).

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