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Recombinant murine interferon gamma ifnγ

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Recombinant murine interferon-gamma (IFNγ) is a cytokine that plays a crucial role in the immune system. It is a protein produced by various cells, including T cells and natural killer cells, and is involved in the regulation of immune responses. This recombinant version of murine IFNγ is produced in a laboratory setting using genetic engineering techniques.

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Lab products found in correlation

Ferric ammonium citrate, by Merck Group (1 mentions) Nih 3t3 mouse fibroblast, by American Type Culture Collection (1 mentions) Hirudin, by Merck Group (1 mentions) Laminin, by Merck Group (1 mentions) Polyclonal rabbit antibody against human fg crossreacts with mouse, by Agilent Technologies (1 mentions) Poly d lysine, by Merck Group (1 mentions) Lps from escherichia coli o111 b4, by Merck Group (1 mentions) Spectinomycin, by Merck Group (1 mentions) Pyrazinamide, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Penicillin, by Sartorius (1 mentions) Streptomycin, by Sartorius (1 mentions) Phosphate buffered saline (pbs), by Sartorius (1 mentions) Sodium pyruvate, by Sartorius (1 mentions) Interleukin 4 il 4, by Thermo Fisher Scientific (1 mentions) L glutamine, by Sartorius (1 mentions) Thapsigargin, by Thermo Fisher Scientific (1 mentions) Anti plcγ1 sc 81, by Santa Cruz Biotechnology (1 mentions) Anti ccr7 apc, by Thermo Fisher Scientific (1 mentions) Ova323 339, by Merck Group (1 mentions)

Spelling variants of this product

Interferon-gamma (14 citations) Murine interferon-gamma (4 citations) Recombinant murine interferon gamma (2 citations) Murine recombinant IFNγ (2 citations)

9 protocols using recombinant murine interferon gamma ifnγ

1

Murine Mammary Carcinoma MM48 Protocol

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Murine mammary carcinoma MM48 was obtained from the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University. The antibodies used in this study are listed in online supplementary table S2. Recombinant murine interferon-gamma (IFNγ) (#315–05) was obtained from PeproTech. Recombinant murine PD-L1 (#cj89) was obtained from NovoProtein. N-[(R)-2-Amino-3-(p-isothiocyanatophenyl)propyl]-trans-(S,S)-cyclohexane-1,2-diamine-N,N,N’,N’’,N’’-pentaacetic acid (p-SCN-CHX-A’’-DTPA, B-355) was purchased from Macrocyclics. N-succinimidyl 3-(tri-n-butylstannyl)benzoate (ATE) was synthesized according to the procedure described previously.12 (link) [In-111]InCl3 (74 MBq/mL, 874300) was purchased from Nihon Medi-Physics. [I-125]NaI (74 MBq, #NEZ033A002MC) was purchased from Perkin Elmer.
Kurino T., Matsuda R., Terui A., Suzuki H., Kokubo T., Uehara T., Arano Y., Hisaka A, & Hatakeyama H. (2020). Poor outcome with anti-programmed death-ligand 1 (PD-L1) antibody due to poor pharmacokinetic properties in PD-1/PD-L1 blockade-sensitive mouse models. Journal for Immunotherapy of Cancer, 8(1), e000400.
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2

Interferon-gamma Supplemented FAC Protocol

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Ferric ammonium citrate (FAC) was purchased from Sigma (USA). Recombinant murine interferon-gamma (IFN-γ) was purchased from PeproTech (USA).
Gan Z.S., Wang Q.Q., Li J.H., Wang X.L., Wang Y.Z, & Du H.H. (2017). Iron Reduces M1 Macrophage Polarization in RAW264.7 Macrophages Associated with Inhibition of STAT1. Mediators of Inflammation, 2017, 8570818.
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3

Isolation and Characterization of Murine Fibroblast and Cardiomyocyte Populations

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NIH/3T3 mouse fibroblasts were purchased from ATCC. The WT19 fibroblasts were a gift from Dr. Anne Campbell (19 (link)). WT19 cells are mouse fibroblasts of the H-2b haplotype obtained from C57Bl/6 mice and are immortalized by transformation with Simian Virus-40 T large antigen. Rat neonatal cardiac fibroblasts were obtained from the pre-plating step during standard isolation of neonatal cardiomyocytes (20 ). To insure absence of contamination with rat cardiomyocytes, the adherent neonatal cardiac fibroblasts were passaged in tissue culture-treated dishes two additional times, following the initial isolation. Mouse stem cell derived cardiac myocytes (mESC-CM) were purchased from LONZA (#XCAC-1010N). The mESC-CM were generated from the ES-D3 mouse embryonic stem cell line (ATCC, 129S4/Jae strain) by stable transduction with a bicistronic vector (21 (link)). This vector contains a cardiac-specific promoter driving the expression of both an antibiotic resistance gene and the EGFP cassette, ensuring selection of a high purity population of mESC-CM. The mESC-CM were maintained and differentiated according to the manufacturer’s instructions. Spontaneously beating mESC-CM layers were used within 3–14 days after cell plating. Where indicated, the cells were treated with 20 ng/mL recombinant murine interferon gamma (IFNγ) (PeproTech, NJ).
Karabekian Z., Idrees S., Ding H., Jamshidi A., Posnack N.G, & Sarvazyan N. (2015). Downregulation of beta-microglobulin to diminish T-lymphocyte lysis of non-syngeneic cell sources of engineered heart tissue constructs. Biomedical materials (Bristol, England), 10(3), 034101.
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4

Astrocyte Activation and ICAM-1 Expression

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Human Fg depleted of plasminogen, von-Willebrand factor, and fibronectin was from Enzyme Research Laboratories (South Bend, IN, USA). Polyclonal rabbit antibody against human Fg (cross-reacts with mouse) was purchased from Dako Cytomation (Carpentaria CA, USA). Rat purified function-blocking antibody (clone: YN1/1.7.4) against mouse ICAM-1 (CD-54, cat. # 116133) was obtained from BioLegend (San Diego, CA, USA) and prion protein (PrPC) blocking peptide (GTX89339-PEP) was from GeneTex (Irvine, CA, USA). For detection of ICAM-1 and PrPC, we used ICAM-1/CD54 antibody raised in mouse (cat. # NBP2-22541) from Novus biologicals (Littleton, CO, USA) and anti-prion protein antibody also raised in mouse (cat. # P0110) from Sigma Aldrich Chemicals Co (St. Louis, MO, USA), respectively. In vitro animal astrocyte medium (AM-a) was purchased from ScienCell Research Laboratories (Carlsbad, CA, USA). Poly-D-Lysine, laminin, hirudin, LPS from Escherichia coli (O111:B4), and DuolinkTM In Situ Detection Reagent Red were from Sigma. Recombinant murine interferon gamma (IFNγ) was purchased from Peprotech (Rocky Hill, NJ, USA).
Sulimai N., Brown J, & Lominadze D. (2021). Fibrinogen Interaction with Astrocyte ICAM-1 and PrPC Results in the Generation of ROS and Neuronal Death. International Journal of Molecular Sciences, 22(5), 2391.
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5

Intranasal Interferon Gamma Dosing in Mice

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Recombinant-murine interferon gamma (IFNγ) (16 ng/g or 60 ng/g, Peprotech, NJ) was administered i.n. to 2–7 day old mice under 2 % isoflurane anesthesia on 1, 3, and 5 days post-infection. The total volume administered to adults was 50 μl and 10–20 μl to neonates. For the pharmacokinetic experiment, a single dose of 16 ng/g was administered i.n. to RSV-naïve mice at time zero and they were subsequently euthanized using 100 % isoflurane at 0, 0.5, 1, 2, 4, 6, 8, 12, 18, 24 and 48 h post-dose.
Eichinger K.M., Egaña L., Orend J.G., Resetar E., Anderson K.B., Patel R, & Empey K.M. (2015). Alveolar macrophages support interferon gamma-mediated viral clearance in RSV-infected neonatal mice. Respiratory Research, 16, 122.
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6

Spectinamide-1599 Synergistic Effects

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Spectinamide-1599 was synthesized as described (Lee et al., 2014 (link)) and provided by Dr. Richard Lee (St. Jude Children’s Research Hospital). Pyrazinamide, spectinomycin and streptomycin were purchased from Sigma-Aldrich (Millipore Sigma, St. Louis, MO, United States) and recombinant murine Interferon Gamma (IFN-γ) from PeproTech (Rocky Hill, NJ, United States). Stock solutions for each drug were prepared as follow; Spectinamide-1599 was diluted at 300 μg/mL with cRPMI Medium. Pyrazinamide was dissolved in cRPMI medium at 20 mg/mL and IFN-γ was dissolved in cRPMI medium at 300 U/mL. On day 2 post-infection cell cultures received drug treatment. Cell cultures were treated with monotherapy or combinations of 200 or 400 μg/mL of PZA, 25 or 100 μg/mL spectinamide-1599 and 50 U/mL murine IFN-γ.
Santos K., Lukka P.B., Grzegorzewicz A., Jackson M., Trivedi A., Pavan F., Chorilli M., Braunstein M., Hickey A., Meibohm B, & Gonzalez-Juarrero M. (2018). Primary Lung Dendritic Cell Cultures to Assess Efficacy of Spectinamide-1599 Against Intracellular Mycobacterium tuberculosis. Frontiers in Microbiology, 9, 1895.
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7

Cellular Oxidative Stress Assay

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Phosphate buffered saline (PBS), Dulbecco's modified Eagle's medium (DMEM), fetal calf serum (FCS) (heat-inactivated at 56°C for 30 min), penicillin, streptomycin, L-glutamine, and sodium pyruvate were from Biological Industries (Beth Haemek, Israel). 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) and lipopolysaccharide (LPS) from Salmonella typhimurium were from Sigma Aldrich (St. Louis, MO, USA). Recombinant murine interferon-gamma (IFNγ) and interleukin-4 (IL-4) were from PeproTech (Rocky Hill, NJ, USA).
Koren-Gluzer M., Rosenblat M, & Hayek T. (2015). Paraoxonase 2 Induces a Phenotypic Switch in Macrophage Polarization Favoring an M2 Anti-Inflammatory State. International Journal of Endocrinology, 2015, 915243.
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8

T cell Receptor Imaging and Activation

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The reagents used in our experiments included: OVA(323–339) (Sigma-Aldrich, St Louis, MO, USA), thapsigargin (TG, stock 1 mM in DMSO, Molecular Probes, Invitrogen, USA), cytochalasin D and nocodazole (Calbiochem, Merck KGaA, Germany). Recombinant murine interferon gamma (IFN-γ) was purchased from Peprotech (Rocky Hill, NJ, USA). For live cell imaging, H57-597-Fab-TCRαβ-Alexa Fluor 647 was purchased from Invitrogen to label TCR as a non-blocking antibody [22 (link)]. For calcium imaging, Calcium Crimson™ was purchased from Invitrogen. The following antibodies (Abs) were used for immunofluorescence: Texas Red-X phalloidin for anti-F-actin, MitoTracker® Green FM (Molecular Probes, Invitrogen, USA), anti-PLCγ1 (sc81, Santa Cruz Biotechnology, Santa Cruz, CA, USA), polyclonal rabbit anti-PKC-θ (sc212, Santa Cruz Biotechnology), anti-ZAP-70 (Y319, Abcam, Cambridge, MA, USA), anti-ORAI1 (ab59330, Abcam, Cambridge, MA, USA) and anti-PMCA (5 F10, Abcam, Cambridge, MA, USA), Dylight 405-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch Laboratories), Alexa Fluor 555-conjugated goat anti-rabbit IgG (Invitrogen). The following Abs were used for flow cytometry: anti-MHC-II (I-Ab)-FITC, antiCD80-PE, anti-CD86-APC and anti-CCR7-APC (all from eBioscience).
Lin W., Suo Y., Deng Y., Fan Z., Zheng Y., Wei X, & Chu Y. (2015). Morphological change of CD4+ T cell during contact with DC modulates T-cell activation by accumulation of F-actin in the immunology synapse. BMC Immunology, 16, 49.
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9

sDR5-Fc Modulates Macrophage Polarization

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sDR5-Fc was presented by En-Yun SHEN, Zhongke Amshenn Pharmaceutical Co. Ltd (Shenzhen, China). Lipopolysaccharide (LPS) was purchased from Sigma (St. Louis, MO, USA). Recombinant murine interferon-gamma (IFN-γ) and TRAIL were purchased from Peprotech (Rocky Hill, NJ, USA). Anti-CD86-FITC antibody was from BD Pharmingen (San Diego, CA, USA). Anti-inducible nitric oxide synthase (iNOS) antibody, anti-peroxisome proliferator-activated receptor gamma (PPARγ) antibody, anti-hexokinase 2 (HK2) antibody, anti-glucose transporter 1 (GLUT1) antibody, were purchased from Abcam (Cambridge, MA, USA). Anti-arginase-1 (Arg-1) antibody was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Anti-β-actin antibody was purchased from Rayantibody (Beijing, China).
ZHAI G.Y., QIE S.Y., GUO Q.Y., QI Y, & ZHOU Y.J. (2021). sDR5-Fc inhibits macrophage M1 polarization by blocking the glycolysis. Journal of Geriatric Cardiology : JGC, 18(4), 271-280.
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