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Mammalian mitochondria isolation kit for tissue cultured cells

Manufactured by Abcam
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The Mammalian Mitochondria Isolation Kit for Tissue & Cultured Cells is a product designed to isolate mitochondria from mammalian tissue and cell culture samples. The kit provides a simple and efficient method to separate mitochondria from other cellular components, enabling the study of mitochondrial structure and function.

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Mitochondria Isolation Kit (63 citations) Mitochondria Isolation Kit for Cultured Cells (29 citations) Mammalian Mitochondria Isolation Kit (4 citations) Cell Mitochondria Isolation Kit (4 citations)

2 protocols using mammalian mitochondria isolation kit for tissue cultured cells

1

Cardiac Mitochondrial Fraction Purification

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The purification of an entire cardiac mitochondrial fraction from the hearts or cultured primary cardiomyocytes was carried out as previously described51 (link), using the Mammalian Mitochondria Isolation Kit for Tissue & Cultured Cells (Biovision, Inc) according to the manufacturer’s instructions.
Yan K., An T., Zhai M., Huang Y., Wang Q., Wang Y., Zhang R., Wang T., Liu J., Zhang Y., Zhang J, & Wang K. (2019). Mitochondrial miR-762 regulates apoptosis and myocardial infarction by impairing ND2. Cell Death & Disease, 10(7), 500.
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2

Mitochondrial ETC Complex Activities Assay

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Electron transport chain (ETC) complexes I and IV activities were measured spectrophotometrically as previously described (27 ). Briefly, brain was resected from mice. Mitochondria were isolated from organs using BioVision Mammalian Mitochondria Isolation Kit for Tissue & Cultured Cells (Catalog #: K288), resuspended in 1 mL KME buffer each, and stored at −80°C. Complex I (NADH dehydrogenase) activity was determined by measuring NADH oxidation at 340nm. Complex IV (cytochrome c oxidase) activity was evaluated by measuring the oxidation of cytochrome c at 550 nm. Each treatment group was comprised of 8 replicate wells in a 96-well plate. Bradford assay was used to measure protein concentration in isolated mitochondria from brain and liver that was used when performing calculations to obtain measurements expressed as nanomoles substrate converted/min/mg of protein. The mean measurement of each treatment group was compared with the mean of the control group (DMSO-only) with a One-Way ANOVA using GraphPad Prism 8 software program following the exclusion of outliers as determined by ROUT (Q = 1%) outlier method.
Saralkar P., Mdzinarishvili A., Arsiwala T.A., Lee Y.K., Sullivan P.G., Pinti M.V., Hollander J.M., Kelley E.E., Ren X., Hu H., Simpkins J., Brown C., Hazlehurst L.E., Huber J.D, & Geldenhuys W.J. (2021). The mitochondrial mitoNEET ligand NL-1 is protective in a murine model of transient cerebral ischemic stroke. Pharmaceutical research, 38(5), 803-817.
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