Rabbit anti inducible nitric oxide synthase

Eyes from OIR and unexposed control mice were fixed and prepared for 10-µm-cross-sectioning. After fixation with 4% paraformaldehyde, permeabilization with 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) and blocking with 5% bovine serum albumin, cross-sections were respectively incubated with rabbit anti-p-IκBα, rabbit anti-IκBα (Abcam, Cambridge, MA, USA), rabbit anti-p-p65, rabbit anti-p65, rabbit anti-inducible nitric oxide synthase (iNOS) (Cell Signaling Technology, Inc., Danvers, MA, USA), and rabbit anti-Arginase-1 (Arg-1) (GeneTex, Irvine, CA, USA) and then incubated with Alexa Fluor 555 anti-rabbit IgG (Cell Signaling Technology) plus Griffonia simplicifolia Isolectin B4 (GSA-Lectin)-labeled fluorescein isothiocyanate (FITC; Vector Laboratories, Inc., Burlingame, CA, USA), PE-F4/80 (eBioscience, Vienna, Austria) plus FITC-GSA-Lectin (Vector Laboratories), or PE-F4/80 plus Alexa Fluor 488 anti-rabbit IgG (Cell Signaling Technology). After incubation with 4′,6-diamidino-2-phenylindole (Beyotime, Shanghai, China), cross-sections were photographed using fluorescence microscopy (Nikon Instruments, Inc., Melville, New York, USA).