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Anti cd11c alexa700 clone 3.9

Manufactured by Thermo Fisher Scientific
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Anti-CD11c Alexa700 (clone 3.9) is a fluorescently labeled monoclonal antibody that binds to the CD11c antigen. CD11c is a cell surface marker expressed on dendritic cells, macrophages, and other myeloid cells. The antibody is conjugated to the Alexa Fluor 700 fluorescent dye, which can be detected using flow cytometry or other fluorescence-based analysis techniques.

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Lab products found in correlation

Alexa fluor 488 antibody, by Thermo Fisher Scientific (2 mentions) Cytofix cytoperm kit, by BD (2 mentions) Anti cd20 pe cf594 clone 2h7, by BD (2 mentions) Anti cd3 bv421 clone sp34 2, by BD (2 mentions) Anti cd16 bv605 clone sg8, by BD (2 mentions) Anti cd4 bv711 clone l200, by BD (2 mentions) Anti cd14 bv786 clone m5e2, by BD (2 mentions) Anti cd123 percp cy5.5 clone 7g3, by BD (2 mentions) Anti cd8 pe cy7 clone sk1, by BD (2 mentions) Anti hla dr apc cy7 clone l243, by BD (2 mentions) Anti p100 clone epr18756, by Abcam (2 mentions) 4 laser fortessa flow cytometer, by BD (2 mentions) Anti cd4 qdot605, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-CD11c (67 citations) CD11c (3.9, (9 citations) Anti-CD11c Alexa700 (5 citations)

3 protocols using anti cd11c alexa700 clone 3.9

1

Multiparameter Flow Cytometric Immune Profiling

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Whole blood collected in Cyto-Chex® blood collection tubes (Myriad RBM) was added to ACK lysis buffer to remove red blood cells, then washed with PBS and resuspended in a staining cocktail including anti-CD3 BV421 (clone SP34-2; BD Biosciences), anti-CD16 BV605 (clone SG8; BD Biosciences), anti-CD4 BV711 (clone L200; BD Biosciences), anti-CD14 BV786 (clone M5E2; BD Biosciences), anti-CD123 PerCP-Cy5.5 (clone 7G3; BD Biosciences), anti-CD20 PE-CF594 (clone 2H7; BD Biosciences), anti-CD8 PE-Cy7 (clone SK1; BD Biosciences), anti-CD11c Alexa700 (clone 3.9; Ebioscience), and anti-HLA-DR APC-Cy7 (clone L243; BD Biosciences). Following surface staining, cells were washed twice with phosphate-buffered saline (PBS), then permeabilized using the Cytofix/Cytoperm kit (BD Biosciences, kit used as directed), and stained intracellularly with anti-p100 (clone EPR18756; Abcam). Cells were washed twice and stained with a secondary chicken anti-rabbit Alexa Fluor 488 antibody (Invitrogen). Samples were acquired using a 4-laser Fortessa™ flow cytometer (BD Biosciences) and analyzed with FlowJo™ software (version 9.7.6, Treestar).
Nixon C.C., Mavigner M., Sampey G.C., Brooks A.D., Spagnuolo R.A., Irlbeck D.M., Mattingly C., Ho P.T., Schoof N., Cammon C.G., Tharp G.K., Kanke M., Wang Z., Cleary R.A., Upadhyay A.A., De C., Wills S.R., Falcinelli S.D., Galardi C., Walum H., Schramm N.J., Deutsch J., Lifson J.D., Fennessey C.M., Keele B.F., Jean S., Maguire S., Liao B., Browne E.P., Ferris R.G., Brehm J.H., Favre D., Vanderford T.H., Bosinger S.E., Jones C.D., Routy J.P., Archin N.M., Margolis D.M., Wahl A., Dunham R.M., Silvestri G., Chahroudi A, & Garcia J.V. (2020). Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo. Nature, 578(7793), 160-165.
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2

Multiparametric Flow Cytometry Panel

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A cocktail composed of the following monoclonal antibodies was used: anti-CD16-FITC (clone 3G8), anti-CD141-PE (clone 1A4), anti-CD123-PerCP-Cy5.5 (clone 7G3), anti-CD3-PE-Cy7 (clone SP34-2), anti-CD14-Pacific Blue (clone M5E2), CD20-APC-Cy7 (clone L27) all from BD Pharmingen (San Jose, CA), anti-CD1c-APC (clone AD5-8E7, Miltenyi Biotec, Auburn, CA), anti-HLA-DR-PE-Texas Red (clone Immu-357, Beckman Coulter, Miami, FL), anti-CD11c-Alexa700 (clone 3.9, eBiosciences, San Diego, CA), anti-CD8-Qdot 655 (clone 3B5, Invitrogen, Carlsbad, CA) and anti-CD4-Qdot 605 (clone S3.5, provided by Dr K. Reimann).
Soulas C., Autissier P.J., Burdo T.H., Piatak M J.r., Lifson J.D, & Williams K.C. (2015). Distinct Phenotype, Longitudinal Changes of Numbers and Cell-Associated Virus in Blood Dendritic Cells in SIV-Infected CD8-Lymphocyte Depleted Macaques. PLoS ONE, 10(4), e0119764.
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3

Multiparameter Flow Cytometric Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole blood collected in Cyto-Chex® blood collection tubes (Myriad RBM) was added to ACK lysis buffer to remove red blood cells, then washed with PBS and resuspended in a staining cocktail including anti-CD3 BV421 (clone SP34-2; BD Biosciences), anti-CD16 BV605 (clone SG8; BD Biosciences), anti-CD4 BV711 (clone L200; BD Biosciences), anti-CD14 BV786 (clone M5E2; BD Biosciences), anti-CD123 PerCP-Cy5.5 (clone 7G3; BD Biosciences), anti-CD20 PE-CF594 (clone 2H7; BD Biosciences), anti-CD8 PE-Cy7 (clone SK1; BD Biosciences), anti-CD11c Alexa700 (clone 3.9; Ebioscience), and anti-HLA-DR APC-Cy7 (clone L243; BD Biosciences). Following surface staining, cells were washed twice with phosphate-buffered saline (PBS), then permeabilized using the Cytofix/Cytoperm kit (BD Biosciences, kit used as directed), and stained intracellularly with anti-p100 (clone EPR18756; Abcam). Cells were washed twice and stained with a secondary chicken anti-rabbit Alexa Fluor 488 antibody (Invitrogen). Samples were acquired using a 4-laser Fortessa™ flow cytometer (BD Biosciences) and analyzed with FlowJo™ software (version 9.7.6, Treestar).
Nixon C.C., Mavigner M., Sampey G.C., Brooks A.D., Spagnuolo R.A., Irlbeck D.M., Mattingly C., Ho P.T., Schoof N., Cammon C.G., Tharp G.K., Kanke M., Wang Z., Cleary R.A., Upadhyay A.A., De C., Wills S.R., Falcinelli S.D., Galardi C., Walum H., Schramm N.J., Deutsch J., Lifson J.D., Fennessey C.M., Keele B.F., Jean S., Maguire S., Liao B., Browne E.P., Ferris R.G., Brehm J.H., Favre D., Vanderford T.H., Bosinger S.E., Jones C.D., Routy J.P., Archin N.M., Margolis D.M., Wahl A., Dunham R.M., Silvestri G., Chahroudi A, & Garcia J.V. (2020). Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo. Nature, 578(7793), 160-165.
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