96 well pcr plates and plate seals

All reagents and chemicals, unless mentioned otherwise, were procured from Sigma-Aldrich (St. Louis, MO). HEPES, pH 7.3, buffer and dimethyl sulfoxide (DMSO) were obtained from Fischer Bioreagents. Extrinsic fluorophore dye, Sypro-orange, was from Invitrogen (Carlsbad, CA). 96-well PCR-plates and plate seals were from Eppendorf (NY, USA). The artificial gene synthesis exercise was carried out by Genscript (Piscataway, NJ). The small molecule library containing oncology drug set III (97 compounds), mechanistic set II (816 compounds) diversity set III (synthetic) (1597 compounds) and natural product set (118 compounds) were obtained from the open chemical repository of the Developmental Therapeutics Program (DTP) of the National Cancer Institute (NCI), National Institutes of Health (NIH) (http://dtp.cancer.gov).

All reagents and chemicals, unless mentioned otherwise, were procured from Sigma-Aldrich (St. Louis, MO) with the following exceptions: HEPES, pH 7.3 buffer was obtained from Fischer Bioreagents and dimethyl sulfoxide (DMSO) from MP Biomedicals LLC. Sypro orange dye was obtained from Invitrogen (Carlsbad, CA). 96-well PCR-plates and plate seals were from Eppendorf (Eppendorf, NY, USA). E. coli dihydrofolate reductase, DHFR, was provided by Prof. Eugene Shakhnovich, Harvard University. The library of small molecules and drugs containing oncology drug set III (97 compounds), mechanistic set II (816 compounds) diversity set III (synthetic) (1597 compounds) and natural product set (118 compounds) were provided by the open chemical repository of Developmental Therapeutics Program (DTP) of the National Cancer Institute (NCI), National Institutes of Health (NIH) (http://dtp.cancer.gov). Furthermore, a set of 400 diverse drug-like and probe-like compounds was provided as 10 mM stock solutions in dimethyl sulfoxide by Medicines for Malaria Venture (MMV) (http://www.mmv.org/malariabox). All provided compounds had been demonstrated to possess antimalarial activity against the blood-stage of P. falciparum and were selected to represent structural diversity, ease of oral absorption and minimum toxicity.