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Anti activated caspase 8

Manufactured by Cell Signaling Technology
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Anti-activated caspase 8 is a lab equipment product that detects the activated form of the caspase 8 enzyme. Caspase 8 is a key mediator of apoptosis, or programmed cell death. This product allows for the identification and quantification of activated caspase 8 in biological samples.

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Lab products found in correlation

Horseradish peroxidase conjugated anti rabbit igg, by Merck Group (2 mentions) Anti activated caspase 3, by Cell Signaling Technology (2 mentions) Anti rabbit igg, by Merck Group (2 mentions) Anti goat igg, by Santa Cruz Biotechnology (2 mentions) Anti β actin, by Merck Group (2 mentions) Anti bax, by Cell Signaling Technology (1 mentions) Anti bcl 2, by Cell Signaling Technology (1 mentions) Anti cyclin b1, by Cell Signaling Technology (1 mentions) Anti total p53, by Cell Signaling Technology (1 mentions) Anti cdk1, by Cell Signaling Technology (1 mentions) Anti tnf α, by Cell Signaling Technology (1 mentions) Anti myeloperoxidase mpo, by Abcam (1 mentions) Anti activated caspase 3, by R&D Systems (1 mentions) In situ cell death detection kit, by Roche (1 mentions) Anti phosphorylated erk, by Cell Signaling Technology (1 mentions) Anti erk, by Cell Signaling Technology (1 mentions) Anti total p38 mapk, by Cell Signaling Technology (1 mentions)

3 protocols using anti activated caspase 8

1

Comprehensive Immunoblotting Approach

Cited 1 time
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Western immunoblots were run as described previously 21 (link). Primary antibodies and their sources were as follows. Anti-total p53, anti-cyclin B1, anti-Bax, anti-Bcl-2, anti-CDK1, anti-phospho-CDK1, anti-activated caspase 8, anti-activated caspase 9, and anti-activated caspase 3 were from Cell Signaling Technology. Anti-ZIKV Env monoclonal antibody was purchased from Zoongen Co., Ltd (Beijing, China). Anti-p21Cip1/Waf1 and anti-6×His tag was from Abcam. Anti-β-actin and the secondary antibodies horseradish peroxidase-conjugated anti-rabbit IgG and anti-rabbit IgG were from Sigma-Aldrich. Anti-goat IgG was from Santa Cruz Biotechnology.
Liu J., Li Q., Li X., Qiu Z., Li A., Liang W., Chen H., Cai X., Chen X., Duan X., Li J., Wu W., Xu M., Mao Y., Chen H., Li J., Gu W, & Li H. (2018). Zika Virus Envelope Protein induces G2/M Cell Cycle Arrest and Apoptosis via an Intrinsic Cell Death Signaling Pathway in Neuroendocrine PC12 Cells. International Journal of Biological Sciences, 14(9), 1099-1108.
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2

Immunohistochemical Analysis of Apoptosis

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Histopathological analysis was performed on formalin-fixed paraffin-embedded tissue using the Tyramide Signal Amplification (TSA) Cy3 system, as recommended by the manufacturer (PerkinElmer), and the following antibodies were used: the primary antibodies anti-Myeloperoxidase (MPO) (Abcam), anti-Tnf-α (Cell Signaling), anti-activated caspase-3 (R&D Systems) and anti-activated caspase-8 (Cell Signaling) and anti-CD324 (E-cadherin) Alexa Fluor 488 (eBioscience), and a biotinylated secondary anti-rabbit antibody (Dianova). Cell death was analysed using the in situ cell death detection kit (Roche) for TUNEL.
Günther C., Buchen B., He G.W., Hornef M., Torow N., Neumann H., Wittkopf N., Martini E., Basic M., Bleich A., Watson A.J., Neurath M.F, & Becker C. (2014). Caspase-8 controls the gut response to microbial challenges by Tnf-α-dependent and independent pathways. Gut, 64(4), 601-610.
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3

Western Blot Analysis of Signaling Proteins

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Western immunoblots were run as described previously [24 (link)]. Primary antibodies and their sources were as follows. Anti-total p38 MAPK, anti-phosphorylated p38 (pp38) MAPK, anti-Erk, anti-phosphorylated Erk, anti-activated caspase 3, anti-activated caspase 8 were from Cell Signaling Technology. Anti-β-actin and the secondary antibodies horseradish peroxidase-conjugated anti-rabbit IgG and anti-rabbit IgG were from Sigma-Aldrich. Anti-goat IgG was from Santa Cruz Biotechnology.
Pei N., Mao Y., Wan P., Chen X., Li A., Chen H., Li J., Wan R., Zhang Y., Du H., Chen B., Jiang G., Xia M., Sumners C., Hu G., Gu D, & Li H. (2017). Angiotensin II type 2 receptor promotes apoptosis and inhibits angiogenesis in bladder cancer. Journal of Experimental & Clinical Cancer Research : CR, 36, 77.
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