Human hemoglobin lyophilized powder

Glycidol (purity 96.0%), fluorescein‐5‐isothiocyanate (FITC purity ≥90.0%), human hemoglobin lyophilized powder, and 1,2‐propanediol (purity 99.5%) were purchased from Sigma‐Aldrich. Acetonitrile and glyceraldehyde (purity 98.0%) were obtained from Tokyo Chemical Industry Co., Ltd., Kanto Chemical Industry Co., Ltd., and Nacalai Tesque, Inc., respectively. Other chemicals (L‐valine, L‐d₈‐valine, sodium chloride, potassium bicarbonate, hydrochloric acid, ethyl acetate, sodium sulfate, toluene, ethyl acetate, ethanol, methanol, ammonium hydroxide, 3‐MCPD (purity 98.0%), epichlorohydrin (purity 99.0%), and acrylic acid (purity 98.0%) were purchased from Wako Pure Chemical Ind., Ltd.

FITC (purity ≥ 90.0%), glycidol (purity 96.0%), human hemoglobin lyophilized powder (Product Number H7379), and human serum albumin lyophilized powder (purity ≥ 96.0%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Acrylamide (purity 95.0%) was obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan). L-Valine-(¹³C₅) (purity 98.0%), used for the synthesis of the glycidol internal standard N-(2,3-dihydroxypropyl)-(¹³C₅)-valine, and L-d8-valine (purity 98.0%), used for the synthesis of the Acrylamide internal standard fluorescein-5-[4-d7-isopropyl-3-(2-carbamoylethyl)-2-thioxo-imidazolidin5-one] (AA-d7-Val-FTH), was obtained from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). All other chemicals and solvents used were of analytical grade.