F 3008

Manufactured by Merck Group

Sourced in Germany

The F-3008 is a laboratory equipment product manufactured by Merck Group. It is a compact and versatile device designed to perform specific functions in a laboratory setting. The core function of the F-3008 is to provide precise and consistent measurements or analyses, as required by the laboratory's needs. No further details on its intended use or capabilities are provided.

Automatically generated - may contain errors

Lab products found in correlation

T4026, by Merck Group (4 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) I80 fluorescence microscope, by Nikon (2 mentions) Nis elements advanced research imaging software, by Nikon (1 mentions) Eclipse 80i microscope, by Nikon (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) 24 well plate, by Corning (1 mentions) Nis elements software, by Nikon (1 mentions) Hoechst 33258, by Thermo Fisher Scientific (1 mentions)

5 protocols using f 3008

Visualizing Microtubules and DNA in DU 145 Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellular microtubules and DNA were visualized in DU 145 cells by indirect immunofluorescence, as previously described.^{14 (link)} The cells were treated with 5 times the IC₅₀ of 1, 2, 3, or 6 or 2 times the IC₅₀ concentration of 4 for 16–18 h; then the cells were fixed with ice-cold methanol, and microtubules were visualized with a β-tubulin antibody (T4026, Sigma- Aldrich) and a FITC-conjugated secondary antibody (F3008, Sigma-Aldrich). The DNA was visualized with 4',6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich). Images were obtained using a Nikon Eclipse 80i microscope and NIS Elements Advanced Research imaging software (Nikon Instruments, Melville, NY, USA).

Shaffer C.V., Cai S., Peng J., Robles A.J., Hartley R.M., Powell D.R., Du L., Cichewicz R.H, & Mooberry S.L. (2016). Texas Native Plants Yield Compounds with Cytotoxic Activities against Prostate Cancer Cells. Journal of natural products, 79(3), 531-540.

+ Open protocol

+ Expand

Microtubule Visualization in HCC1937 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HCC1937 breast cancer cells (ATCC) were plated on coverslips and allowed to adhere overnight after which they were treated with compounds or vehicle for 4 h at concentrations that caused 75% cytotoxicity of cells (LC₇₅) in the 48 h SRB assay. Cells were then fixed with cold methanol and microtubules visualized by immunofluorescence using a β-tubulin primary antibody (Sigma T-4026, 1:500) and FITC-conjugated secondary antibody (Sigma F-3008, 1:500) along with DAPI staining. Images were acquired on a Nikon i80 fluorescence microscope by compressing multiple y-stacked images using NIS elements software.

Risinger A.L., Hastings S.D, & Du L. (2021). Taccalonolide C-6 Analogues, Including Paclitaxel Hybrids, Demonstrate Improved Microtubule Polymerizing Activities. Journal of natural products, 84(6), 1799-1805.

+ Open protocol

+ Expand

Immunofluorescent Visualization of Microtubules

Check if the same lab product or an alternative is used in the 5 most similar protocols

BMDM cells grown on coverslips were fixed and permeabilized using cold 100% methanol and immunostained using anti–β-tubulin (1:400 dilution, T4026; Sigma Aldrich) as primary and anti-mouse FITC (1:200 dilution, F-3008; Sigma Aldrich) as a secondary antibody. HCC1937 cells were stained with 50 nM Mitotracker Red CMXRos (Thermo-Fisher) in serum-free RPMI 1640 for 30 minutes at 37°C, fixed with 4% paraformaldehyde, and permeabilized with 0.5% (v/v) Triton X-100 prior to immunostaining with anti–β-tubulin. Samples were imaged on a Nikon (Tokyo, Japan) Eclipse 80i fluorescence microscope with NIS elements using z-stacks at 1000× total magnification.

Fermaintt C.S., Takahashi-Ruiz L., Liang H., Mooberry S.L, & Risinger A.L. (2021). Eribulin Activates the cGAS-STING Pathway via the Cytoplasmic Accumulation of Mitochondrial DNA. Molecular Pharmacology, 100(4), 309-318.

+ Open protocol

+ Expand

Immunofluorescence Imaging of Microtubules

Check if the same lab product or an alternative is used in the 5 most similar protocols

HCC1937 cells were seeded on coverslips in 24-well plates (Corning) and left to adhere overnight. Cells were treated with either 100 nM zampanolide or DMSO vehicle control for 18 h and then fixed with 100% methanol for 5 min. Indirect immunofluorescence was used to detect β-tubulin using a primary (Sigma-Aldrich, T-4026, 1:400) and a secondary conjugated to FITC (Sigma-Aldrich, F-3008, 1:200) and stained with 0.1 μg/mL DAPI (Sigma-Aldrich). Images were acquired on an i80 fluorescence microscope (Nikon, Tokyo, Japan) by compressing multiple y-stacked images using NIS elements software (Nikon).

Takahashi-Ruiz L., Morris J.D., Crews P., Johnson T.A, & Risinger A.L. (2022). In Vivo Evaluation of (−)-Zampanolide Demonstrates Potent and Persistent Antitumor Efficacy When Targeted to the Tumor Site. Molecules, 27(13), 4244.

+ Open protocol

+ Expand

BACH1 Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed in 4% formaldehyde/PBS. After incubation with anti-BACH1 (1:200, 9D11) antibodies for 1 h at 37 °C, the antigen-antibody complexes were detected by an anti-mouse IgG FITC-conjugated secondary antibody (1:1000, F3008, Sigma Aldrich, Darmstadt, Germany). Hoechst 33258 (Thermo Fisher, Waltham, MA, USA) was used at 20 µg/mL to stain the nuclei.

Liu L., Matsumoto M., Matsui-Watanabe M., Ochiai K., Callens B.K., Nguyen L.C., Kozuki Y., Tanaka M., Nishizawa H, & Igarashi K. (2022). BACH1 Expression Is Promoted by Tank Binding Kinase 1 (TBK1) in Pancreatic Cancer Cells to Increase Iron and Reduce the Expression of E-Cadherin. Antioxidants, 11(8), 1460.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!