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Mc 21

Manufactured by BioXCell
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Sourced in United Kingdom

The MC-21 is a compact and versatile laboratory centrifuge designed for a wide range of applications. It features a high-speed motor and a durable construction, providing reliable and consistent performance. The MC-21 is suitable for various sample preparation and separation tasks, ensuring efficient and reproducible results.

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Lab products found in correlation

Pk136, by BioXCell (1 mentions) Sch58261, by Merck Group (1 mentions) Rmil 4, by BioLegend (1 mentions) Rmgm csf, by Merck Group (1 mentions) Rmil 33, by BioLegend (1 mentions) Trfk5, by BioXCell (1 mentions) Jes5 2a5, by BioXCell (1 mentions) Rat igg2a, by BioXCell (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

2 protocols using mc 21

1

Radiation-Induced Myeloablation and Bone Marrow Transplantation

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Anaesthetized 6–8-week old C57BL/6J CD45.1+CD45.2+ animals were exposed to a single dose of 9.5 Gy γ-irradiation while all but the hind legs and lower abdomen, or where specified, all but the head and upper thorax of the animals, were protected by a 2 inch lead shield. Animals were subsequently given 2-5 × 106 BM cells from CD45.2+ C57BL/6J or Ccr2−/− animals by iv injection. In certain experiments, BM cells were given together with 20 μg of anti-CCR2 mAb (MC-21)32 (link) or rat IgG2b control mAb (BioXcell, UK), or with PBS alone, and efficacy of anti-CCR2 mAb treatment determined by FACS analysis on 10 μl tail venous blood samples 24 h later. Unless specified, mice were left for a period of 8 weeks before analysis of chimerism in the tissue compartments.
Bain C.C., Hawley C.A., Garner H., Scott C.L., Schridde A., Steers N.J., Mack M., Joshi A., Guilliams M., Mowat A.M., Geissmann F, & Jenkins S.J. (2016). Long-lived self-renewing bone marrow-derived macrophages displace embryo-derived cells to inhabit adult serous cavities. Nature Communications, 7, ncomms11852.
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2

Murine Allergic Airway Inflammation Model

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Mice were anesthetized by isofluorane inhalation, followed by the intranasal administration of rmIL-33 (0.2 µg, Biolegend), rmIL-4 (0.5 µg), rmIL-13 (0.5 µg), rmGM-CSF (0.5 µg), rmIL-5 (0.5 µg), LPS (1 μg, Sigma), CpG (10 μg, Invivogen), Aspergillus protease allergen (0.01U, Sigma), Ragweed pollen extract (300 μg, Greer) or clodronate liposome (C.L.) (30% C.L./PBS, Liposoma B.V.) in 40 µl of PBS. Diphtheria toxin (10 ng/g, Sigma), IL-33R-Fc (10 mg/kg, AstraZeneca), anti-NK1.1 mAb (50 μg, PK136, BioXcell), anti-CCR2 (20 μg, MC-21, provided by Prof. Matthias Mack52 (link)), anti-CCL2 (200 μg, MCP-1, BioXcell), anti-Ly6C/G (200 μg, GR-1, BioXcell), anti-Ly-6G (200 μg, 1A8, BioXcell), anti-CD4 (100 μg, GK1.5, BioXcell), anti-IL-10 (300 μg, JES5-2A5, BioXcell), anti-TGF-β (400 μg, 1D11.16.8, BioXcell), anti-IL-5 (100 μg, TRFK5, BioXcell), rat IgG1, κ (BioXcell), rat IgG2a (BioXcell), or rmIL-33 (0.5 µg, Biolegend) was administered by intraperitoneal injection in 100 µl of PBS. A2AR antagonist (20 μg, SCH 58261, Sigma) was administered in DMSO/PBS (v/v).
Schuijs M.J., Png S., Richard A.C., Tsyben A., Hamm G., Stockis J., Garcia C., Pinaud S., Nicholls A., Ros X.R., Su J., Eldridge M.D., Riedel A., Serrao E.M., Rodewald H.R., Mack M., Shields J.D., Cohen E.S., McKenzie A.N., Goodwin R.J., Brindle K.M., Marioni J.C, & Halim T.Y. (2020). ILC2-driven innate immune checkpoint mechanism antagonizes NK cell anti-metastatic function in the lung. Nature immunology, 21(9), 998-1009.
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