Cells were seeded at 200,000 cells per well of 6 well dishes on the day prior to treatment and adhered overnight. For time course experiments, drugs were added in a manner such that all samples were collected at the same time. Post treatment, cells were collected and fixed with 4% formaldehyde for 15 minutes. After two washes with PBS, cells were exposed to 100% methanol at −20°C for >2 hours. Cells were washed with PBS and incubated with the active caspase-3 antibody (1:500 dilution, BD Biosciences) in a 50/50 (v/v) PBS-T:Odyssey blocking buffer solution (LICOR). Cells were washed with PBS-T and incubated with the Alexa-fluor 647 cleaved PARP primary and Alexa-fluor 488 goat anti-rabbit secondary antibodies (1:500 dilution, BD Bioscience) overnight at room temperature. FACS samples were run on an LSR II machine with excitation lasers of 488 and 640 nm.
Alexa fluor 647 cleaved parp primary and alexa fluor 488 goat anti rabbit secondary antibodies
Manufactured by BD
Alexa-fluor 647 cleaved PARP primary and Alexa-fluor 488 goat anti-rabbit secondary antibodies are fluorescently labeled antibody reagents. The Alexa-fluor 647 primary antibody binds to cleaved PARP, while the Alexa-fluor 488 secondary antibody binds to the primary antibody. These reagents are designed for use in immunofluorescence applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alexa fluor 647 cleaved parp primary and alexa fluor 488 goat anti rabbit secondary antibodies
1
Apoptosis Assessment by Flow Cytometry
2
Apoptosis Assessment by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded at 200,000 cells per well of 6 well dishes on the day prior to treatment and adhered overnight. For time course experiments, drugs were added in a manner such that all samples were collected at the same time. Post treatment, cells were collected and fixed with 4% formaldehyde for 15 minutes. After two washes with PBS, cells were exposed to 100% methanol at −20°C for >2 hours. Cells were washed with PBS and incubated with the active caspase-3 antibody (1:500 dilution, BD Biosciences) in a 50/50 (v/v) PBS-T:Odyssey blocking buffer solution (LICOR). Cells were washed with PBS-T and incubated with the Alexa-fluor 647 cleaved PARP primary and Alexa-fluor 488 goat anti-rabbit secondary antibodies (1:500 dilution, BD Bioscience) overnight at room temperature. FACS samples were run on an LSR II machine with excitation lasers of 488 and 640 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!