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2 protocols using deuterium oxide 99.90 d

1

Carbamoyl Phosphate Synthesis Protocol

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The following chemical compounds were purchased from commercial suppliers and used without further purification: carbamoyl phosphate disodium salt (Sigma-Aldrich Co., cat. n°C4135-1G), ammonia 28% analaR Normapur (VWR Chemicals, cat n°21190.292), sodium cyanate (Sigma-Aldrich Co., cat. n°185086-100G), trisodium trimetaphosphate (Sigma-Aldrich Co., cat. n°T5508-500G), ammonium carbamate (Sigma-Aldrich Co., cat. n°292834-100G), urea ACS reagent (Sigma-Aldrich Co., cat. n°U5128-100G), ammonium carbonate ACS reagent (Aldrich chemical company, Inc., cat. n°20,786-1), deuterium oxide 99.90% D (Eurisotop, cat. n°D214FE).
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2

Synthesis and Characterization of Ureidosuccinic Acid Derivatives

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The following chemical compounds were purchased from commercial suppliers and used without further purification: ureidosuccinic acid (Sigma-Aldrich Co., cat. n°69037-500MG), biuret (Sigma-Aldrich Co., cat. n°15270-25G), L-aspartic hemimagnesium salt dihydrate (Sigma-Aldrich Co., cat. n°11260-100G), L-aspartic acid (Sigma-Aldrich Co., cat. n°A8949-100G), magnesium carbonate basic (Sigma-Aldrich Co., cat. n°13118-1 KG) with a Brunauer–Emmett–Teller surface area of 31.5 m2/g, sodium cyanate (Sigma-Aldrich Co., cat. n°185086-100G), sodium hydroxide anhydrous pellets (Carlo Erba reagents, cat. n°480507), deuterium oxide 99.90% D (Eurisotop, cat. n°D214FE), linear dimer H-Asp-Asp-OH (Bachem, cat. n°4010210.0250), ammonium carbamate (Sigma-Aldrich Co., cat. n°292834-100G), ammonia 28% analaR Normapur (VWR Chemicals, cat n°21190.292), trisodium trimetaphosphate (Sigma-Aldrich Co., cat. n°T5508-500G), urea ACS reagent (Sigma-Aldrich Co., cat. n°U5128-100G), ammonium carbonate ACS reagent (Aldrich chemical company, Inc., cat. n°20786-1), carbamoyl phosphate disodium salt (Sigma-Aldrich Co., cat. n°C4135-1G), fumed silica Aerosil 380 (Evonik Industries), with a Brunauer–Emmett–Teller surface area of 380 m2/g.
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