Procaine hcl

Procainamide, lot #54F-0048, procaine-HCl, lot #125K0697, isoproterenol, D-amphetamine, L-DOPA, lot #077K1844, and 5-atenolol, lot #044K3485, were purchased from Sigma-Aldrich Corporation, St. Louis, MD. APTT reagent, lot #2006-02-02/527313A, 0.025  $\underset{\_}{\underset{\_}{\text{M}}}$ CaC1₂, lot #05-17-2004/5006872, Ci-Trol coagulation control, Level I, lots #2009-02-05/508114, 2006-01-19/538162/245, and 2010-06-07/548137/255, were obtained from Dade Behring, Marburg. Assess TM Level I plasma lot #N1106614 was obtained from Instrumentation Laboratory Company, Lexington, MA. Brockman I aluminum oxide, lot #0791DY, was purchased from Aldrich Chemical Company, Milwaukee, WI. Acetaldehyde (AcH), 99%, batch #00339MB, was secured from Sigma-Aldrich and passed through short columns of aluminum oxide in order to remove oxidation products. It was subsequently stored under N₂ at −20°C until further use.
APTT assays were carried out as originally described elsewhere [6 (link)]. A fibrosystem fibrometer precision coagulation timer, model 5, Becton, Dickinson and Company, Cockeysville, MD, was employed to assay the APTTs.

Reagents were obtained from the following suppliers: 9-aminoacridine (9-AAA), ethidium bromide, diacetate fluorescein, Alexa Fluor 488 Phalloidin, gentamicin, calcein, Lucifer yellow, phenol red; toluidine blue, lidocaine HCl, procaine HCl, tetracaine HCl and trypsin-EDTA from Sigma; fetal bovine serum (FBS) from Gibco, Invitrogen; carboxyfluorescein from Fluka-biochemist; culture medium RPMI 1640 with L-glutamine from Lonza; NaCl and sucrose from Merck; and phosphate-buffered saline (PBS) without calcium and magnesium ions and with calcium and magnesium ions from Biomed.