L. biflexa sevorar Patoc strain Patoc (Paris) and bioluminescent derivative PFlum7, L. interrogans Icterohaemorraghiae strain Verdun, L. interrogans serovar Copenhageni strain Fiocruz L1-130, L. interrogans serovar Manilae strain L495 and bioluminescent derivative strain MFLum1 were described earlier [7 (link), 9 (link), 10 (link)]. Bacteria were grown in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium (Bio-Rad) at 28°C without agitation.
Emjh medium
Manufactured by Bio-Rad
EMJH medium is a laboratory culture medium used for the growth and isolation of microorganisms, specifically Leptospira bacteria. The medium provides the necessary nutrients and growth factors to support the cultivation of Leptospira species. It is a commonly used tool in microbiology and infectious disease research.
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Lab products found in correlation
2 protocols using emjh medium
1
Leptospira Cultivation and Bioluminescent Strains
2
Propagation and Maintenance of Leptospira
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L. biflexa sevorar Patoc strain Patoc (Paris) and L. interrogans serovar Manilae strain L495 were used in this study as described earlier [17] (link). Bacteria were grown in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium (Bio-Rad) at 28°C without agitation. EMJH agar plates were obtained by solidification of EMJH medium with 1.2% noble agar (Difco). Leptospires were counted using a Petroff-Hauser chamber. Antibiotics were used in vitro at the following concentrations: 100 µg/mL ampicillin (Amp, MP Biomedical); 25 µg/ml kanamycin (Km, Sigma-Aldrich); 50 µg/ml spectinomycin (Spc, Sigma-Aldrich). Thymidine (dT, Sigma-Aldrich) and diaminopimelate (DAP, Sigma-Aldrich) were added when necessary at the final concentration of 0.3 mM.
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L. biflexa sevorar Patoc strain Patoc (Paris) and L. interrogans serovar Manilae strain L495 were used in this study as described earlier [17] (link). Bacteria were grown in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium (Bio-Rad) at 28°C without agitation. EMJH agar plates were obtained by solidification of EMJH medium with 1.2% noble agar (Difco). Leptospires were counted using a Petroff-Hauser chamber. Antibiotics were used in vitro at the following concentrations: 100 µg/mL ampicillin (Amp, MP Biomedical); 25 µg/ml kanamycin (Km, Sigma-Aldrich); 50 µg/ml spectinomycin (Spc, Sigma-Aldrich). Thymidine (dT, Sigma-Aldrich) and diaminopimelate (DAP, Sigma-Aldrich) were added when necessary at the final concentration of 0.3 mM.
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