Nitrone spin trap phenyl n tert butylnitrone

Analytical grade chemicals nitrone spin trap phenyl-N-tert-butylnitrone (PBN), tert-butylbenzene, (2,2,6,6-tetramethyl-piperidin-1-yl)oxyl (TEMPO), and 1-λ1-oxidanyl-2,2,6,6-tetramethyl-piperidin-4-ol (TEMPOL) were obtained from Sigma-Aldrich (St. Louis, MO, U.S.). Different types of activated charcoal (A = anasorb 747, B = CSC, and C = JXC (SKC Inc., Eighty Four, PA, U.S., material characteristics provided in Supporting Information Table S1)), Suprasil EPR tubes (4 mm o.d.; Wilmad-Labglass,Vineland, NJ, U.S.), Schlenk line (Chemglass Life Sciences, Vineland, NJ, U.S.), 500–750 μm glass beads (Fisher Scientific, PA, U.S.), and Cambridge filters pads (CFP, Performance Systematix Inc., Grand Rapids, MI, U.S.) were used as supplied. Cigarette holders (Hleeduo ZB-033) were purchased online from Amazon.

The 3R4F research cigarette was obtained from the University of Kentucky (Lexington, Kentucky, USA). All the commercial cigarettes were purchased locally (Hershey, PA): 26 King size (85 mm) and Virginia Slims Gold (100 mm). The cigarette brand varieties were selected based on their market share or unique characteristics. Marlboro, Newport, Pall Mall and Camel were selected for their popularity, with variants of these brands comprising an estimated total US market share of 66%.³¹ Parliament and American Spirit exemplify the types of unique characteristics such as recessed filters and “additive free” formulation, respectively. The cigarettes were stored in their original packaging long term at −20°C in airtight plastic bags. Analytical grade chemicals: nitrone spin trap phenyl-N-tert-butylnitrone (PBN), tert-butylbenzene, 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO), 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL), heptadecane from Sigma-Aldrich (St. Louis, MO, USA), Suprasil® EPR tubes (4mm o.d;Wilmad-Labglass,Vineland, NJ, USA), Schlenk line (Chemglass Life Sciences,Vineland, NJ, USA) and Cambridge filters pads (Performance Systematix Inc.,Grand Rapids, MI, USA) were used as supplied.