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Male dba 2j mice

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Male DBA/2J mice are a common inbred mouse strain used in laboratory research. They serve as a standard laboratory animal model for various studies.

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Onetouch ultra 2 blood glucose monitoring system, by LifeScan (2 mentions) C57bl 6j female, by Jackson ImmunoResearch (1 mentions) Lab diet 5001, by Land O'Lakes (1 mentions)

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DBA/2J (81 citations) DBA/2J mice (72 citations) DBA/2J males (2 citations)

15 protocols using male dba 2j mice

1

Male Mouse Acclimation and Behavioral Experiments

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DBA/2J male mice, 6 to 7 weeks old upon arrival to the laboratory, were obtained from Jackson Laboratory (Bar Harbor, Maine, USA) and acclimated for 2 weeks before experiments started. Male mice were used here to facilitate comparison to previous studies of extinction (Groblewski et al. 2011 (link)) and NLX (Cunningham et al. 1995 , 1998 (link)), which involved male mice only. Mice were housed four per cage in a Thoren rack with tap water and standard rodent chow available ad libitum. The colony room was maintained at a temperature of 21±1°C on a 12 h light/dark cycle (lights on at 7:00 a.m.). All procedures were approved by the OHSU Institutional Animal Care and Use Committee and were carried out in accordance with National Institutes of Health Principles of Laboratory Animal Care.
Font L., Houck C.A, & Cunningham C.L. (2017). Naloxone effects on extinction of ethanol- and cocaine-induced conditioned place preference in mice. Psychopharmacology, 234(18), 2747-2759.
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2

Breeding C57BL/6J and DBA/2J Mice

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C57BL/6J female and DBA/2J male mice were purchased from the Jackson Laboratory (Bar Harbor, ME) for breeding. All mice were housed in individually ventilated cages at a constant temperature (24-26°C) and under a controlled light cycle (12h light and 12h of dark) with food and water provided ad libitum. All animal use protocols were approved by the ‘Institutional Animal Care and Use Committee’ (IACUC) at the University of Georgia and the experiments were conducted in accordance with all specified guidelines.
Yang L., Baumann C., De La Fuente R, & Viveiros M.M. (2020). Mechanisms underlying disruption of oocyte spindle stability by bisphenol compounds. Reproduction (Cambridge, England), 159(4), 383-396.
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3

Husbandry of DBA/2J Male Mice

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Six-week old male DBA/2J male mice were obtained from the Jackson Laboratory 2–3 weeks before experiments began. Mice were group housed (4/cage) in a ventilated Thoren rack with water and rodent chow available at all times. The colony room was maintained on a 12 h light/dark cycle (lights on at 7:00 a.m.) at a temperature of 21±1°C. All procedures were carried out in accordance with National Institutes of Health Principles of Laboratory Animal Care and were approved by the OHSU Institutional Animal Care and Use Committee.
Cunningham C.L., Bakner L., Schuette L.M, & Young E.A. (2020). Morphine and ethanol pretreatment effects on expression and extinction of ethanol-induced conditioned place preference and aversion in mice. Psychopharmacology, 238(1), 55-66.
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4

Behavioral Testing of Male DBA/2J Mice

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Male DBA/2J mice were purchased from The Jackson Laboratory (Sacramento, CA) and housed 2-4 per cage. All mice were allowed to acclimate for at least 2 weeks after arrival before behavioral testing, which began when mice were 55-60 days old. All mice were maintained in standard mouse shoebox cages (28.5 L × 17.5 W × 12 H cm) lined with Bed-o'Cobs® bedding (The Andersons, Inc., Maumee, OH, USA) and had ad libitum access to water and food (LabDiet® 5001, PMI Nutrition International LLC, St. Louis, MO, USA) that was purchased from Animal Specialties Inc. (Hubbard, OR, USA). All mice were experiment- and drug-naïve prior to testing, and behavioral testing was conducted during the light phase of the 12:12 h light:dark cycle (lights on at 0600 h), between 0800 and 1600 h. All CPP conditioning and preference test sessions were run Monday through Friday.
Gubner N.R., Cunningham C.L, & Phillips T.J. (2015). Nicotine Enhances the Locomotor Stimulating but Not the Conditioned Rewarding Effect of Ethanol in DBA/2J mice. Alcoholism, clinical and experimental research, 39(1), 64-72.
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5

Doxycycline Effects on Diabetic Mice

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Male DBA/2J mice, 10–11 weeks of age, (The Jackson Laboratories, Bar Harbor, Maine) were treated either with streptozotocin (STZ) to induce diabetes (40 mg/kg/day × 5 days) or with vehicle (100 mM citrate, pH 4.5). At ~ 12–13 weeks of age, diabetic mice, as confirmed by glucosuria, were then randomly assigned to treatment with doxycycline (DOX) rodent chow (STZ + DOX: Bio-Serv product #S3888, doxycycline concentration of 200 mg/kg, Frenchtown, NJ; n = 20) or with a control (CON) chow for doxycycline diets (STZ + CON: Bio-Serv product #S4207; n = 20). Vehicle-treated (non-diabetic) mice were similarly fed, receiving either doxycycline chow (VEH + DOX; n = 10) or control chow (VEH + CON; n = 10). Mice were provided ad libitum access to water and to their assigned food. Food consumption and body weight (Table 1) were measured weekly for the next 10 weeks. Blood glucose was measured on trunk blood at sacrifice via glucometer (OneTouch® Ultra®2 Blood Glucose Monitoring System, LifeScan, Inc., Milpitas, CA; average intra-assay coefficient of variation of 1.7% across a range of 40–300 mg/dl target glucose concentrations). All procedures were approved by the Institutional Animal Care and Use Committee at the University of Arkansas for Medical Sciences.
Fowlkes J.L., Nyman J.S., Bunn R.C., Cockrell G.E., Wahl E.C., Rettiganti M.R., Lumpkin CK J.r, & Thrailkill K.M. (2015). Effects of long-term doxycycline on bone quality and strength in diabetic male DBA/2J mice. Bone Reports, 1, 16-19.
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6

Ethanol-Induced Conditioned Place Preference in DBA/2J Mice

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Male DBA/2J mice (n = 123; The Jackson Laboratory, Sacramento, CA)
6–7 weeks of age upon arrival were received in 4 separate shipments (n =
32–36/shipment). This inbred strain was chosen based on evidence from
our laboratory showing that DBA/2J mice consistently develop robust
ethanol-induced CPP [19 (link)]. Mice were
housed in polycarbonate cages (2–4 per cage) lined with cob bedding in a
colony room maintained at 21+/−1C on a 12:12 h light-dark cycle with
lights on at 7:00 am. All procedures were conducted during the light phase (7:00
am – 7:00 pm). Mice were given approximately 1 week to acclimate to the
colony before surgery. During this time, mice were housed in groups of four.
After surgeries, mice were housed 2 per cage to reduce headmount damage and
cannula loss from allogrooming. Home cage access to lab chow (5L0D
PicoLab® Rodent Diet, St. Louis, MO) and water was provided ad
libitum. Procedures were approved by the Oregon Health &
Science University Institutional Animal Care and Use Committee and carried out
in compliance with the National Institutes of Health Guide For the Care and Use
of Laboratory Animals (NIH Publications No. 8023, revised 2011).
Pina M.M, & Cunningham C.L. (2016). Involvement of ventral tegmental area ionotropic glutamate receptors in the expression of ethanol-induced conditioned place preference. Behavioural brain research, 313, 23-29.
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7

Male DBA/2J Mice Housing and Surgery

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Male DBA/2J mice (6–10 weeks on arrival; Jackson Labs, Bar Harbor, ME) were housed in polycarbonate home cages with rodent bedding. The vivarium was on a reverse light cycle (14:10) with lights off from 09:00–19:00. Food and water were available ad libitum except as stated during food training. Mice were group housed until surgery, and were individually housed with a cotton nestlet (Shred-A-Bed) after surgery. All protocols were approved by the UTHSCSA Animal Care and Use Committee (Protocol 12066x), and mice were cared for consistent with The Guide for the Care and Use of Laboratory Animals. Humane endpoints (loss of >20% body weight, infection, etc) were in place for guiding decisions on the need for euthanasia throughout the course of the experiments. For surgery, mice were anesthetized with isoflurane and were pro-actively treated for possible pain and infection post-surgery with carprofen and ticarcillin.
Sharpe A.L., Varela E, & Beckstead M.J. (2017). Systemic PD149163, a neurotensin receptor 1 agonist, decreases methamphetamine self-administration in DBA/2J mice without causing excessive sedation. PLoS ONE, 12(7), e0180710.
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8

Mouse Housing and Care Protocol

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Male DBA/2J mice (n=116; The Jackson Laboratory, Sacramento, CA) were 7 weeks old at arrival. Mice were housed 4/cage in a colony room maintained at 21±1°C set to a 12:12 light-dark cycle (lights on at 07:00 am). Home cage access to food and water was provided ad libitum throughout all experiments. All procedures complied with the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 8023, revised 2011) and were approved by Oregon Health & Science University’s Institutional Animal Care and Use Committee and Institutional Biosafety Committee.
Pina M.M, & Cunningham C.L. (2016). Ethanol-Seeking Behavior is Expressed Directly through an Extended Amygdala to Midbrain Neural Circuit. Neurobiology of learning and memory, 137, 83-91.
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9

Male DBA/2J Mice Acclimation

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Male DBA/2J mice were obtained from Jackson laboratories at 6 weeks of age and allowed to acclimate to the animal facility at the UNC Thurston-Bowles Center for 1 week with ad libitum access to food and water before experiments began.
Marcinkiewcz C.A., Dorrier C.E., Lopez A.J, & Kash T.L. (2014). Ethanol induced adaptations in 5-HT2c receptor signaling in the bed nucleus of stria terminalis: Implications for anxiety during ethanol withdrawal. Neuropharmacology, 89, 157-167.
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10

PCP-Induced Behavioral Changes in Mice

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Male DBA/2J mice (Jackson Laboratory) were group-housed on a reverse cycle (lights off 0900-1900), with food and water available ad libitum. Treated mice received PCP (5 mg/kg, i.p.) or saline every twelve hours for seven consecutive days, followed by seven days with no treatment. All experiments were reviewed and approved by the UT Health, San Antonio Institutional Animal Care and Use Committee.
Piccart E., Tschumi C.W, & Beckstead M.J. (2019). Acute and subchronic PCP attenuate D2 autoreceptor signaling in substantia nigra dopamine neurons. European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, 29(3), 444-449.
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