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Pre designed primers

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Pre-designed primers are short DNA sequences used in molecular biology techniques, such as PCR (Polymerase Chain Reaction), to identify and amplify specific target regions of genetic material. They serve as the starting point for DNA synthesis, enabling the selective replication of target sequences.

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Designed primers (4 citations) Pre-designed primer/probe mixes (2 citations)

2 protocols using pre designed primers

1

Copy Number Variant Quantification

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For 36 PZMs in Group C where there are copy number variants in the Database of Genomic Variants (http://dgv.tcag.ca/dgv/app/home) that are within 2kb of the PZMs, we selected pre-designed primers from ThermoFisher that assay the copy number variants. The DNA samples used for these quantitative PCR assays were extracted from whole blood samples from the Simons Simplex Collection, and the quantitative PCR assays were performed by the Biopolymers core facility at Harvard Medical School. The reference assay used was AGO1.
Lim E.T., Uddin M., De Rubeis S., Chan Y., Kamumbu A.S., Zhang X., D'Gama A., Kim S.N., Hill R.S., Goldberg A.P., Poultney C., Minshew N.J., Kushima I., Aleksic B., Ozaki N., Parellada M., Arango C., Penzol M.J., Carracedo A., Kolevzon A., Hultman C.M., Weiss L.A., Fromer M., Chiocchetti A.G., Freitag C.M., Church G.M., Scherer S.W., Buxbaum J.D, & Walsh C.A. (2017). Rates, Distribution, and Implications of Post-zygotic Mosaic Mutations in Autism Spectrum Disorder. Nature neuroscience, 20(9), 1217-1224.
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2

Copy Number Variant Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
For 36 PZMs in Group C where there are copy number variants in the Database of Genomic Variants (http://dgv.tcag.ca/dgv/app/home) that are within 2kb of the PZMs, we selected pre-designed primers from ThermoFisher that assay the copy number variants. The DNA samples used for these quantitative PCR assays were extracted from whole blood samples from the Simons Simplex Collection, and the quantitative PCR assays were performed by the Biopolymers core facility at Harvard Medical School. The reference assay used was AGO1.
Lim E.T., Uddin M., De Rubeis S., Chan Y., Kamumbu A.S., Zhang X., D'Gama A., Kim S.N., Hill R.S., Goldberg A.P., Poultney C., Minshew N.J., Kushima I., Aleksic B., Ozaki N., Parellada M., Arango C., Penzol M.J., Carracedo A., Kolevzon A., Hultman C.M., Weiss L.A., Fromer M., Chiocchetti A.G., Freitag C.M., Church G.M., Scherer S.W., Buxbaum J.D, & Walsh C.A. (2017). Rates, Distribution, and Implications of Post-zygotic Mosaic Mutations in Autism Spectrum Disorder. Nature neuroscience, 20(9), 1217-1224.
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