Polyadenylic acid potassium salts

Tris and diethypyrocarbonate were purchased from Amresco (Solon, OH, USA). Mouse anti-Flag-tag monoclonal antibody, anti-Flag M2-agrarose, protease inhibitor cocktail, leupeptin, phenylmethylsulfonyl fluoride (PMSF), Triton X-100, Tween-20, Nonidet P40 (NP-40), hydroxyl urea (HU), ribonuclease A (RNase A), bovine serum albumin (BSA), paraformaldehyde, imidazole, sodium dodecyl sulfate (SDS), guanidine hydrochloride (GdnHCl), polyadenylic acid potassium salts (catalog number: P9403-25MG), DTT, kanamycin, ampicillin and isopropyl-1-thio-β-D-galactopyranoside (IPTG) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-PARN and mouse anti-nucleophosmin (NPM1) antibodies were from Abcam (Cambridge, MA, USA). FITC AffiniPure goat anti-mouse IgG, FITC AffiniPure goat anti-rabbit IgG, mouse anti-HA, rabbit anti-HA, DyLight 594 AffiniPure goat anti-mouse, DyLight 594 AffiniPure goat anti-rabbit, HRP AffiniPure goat anti-mouse and HRP AffiniPure goat anti-rabbit antibodies were from EarthOx (Millbrae, CA, USA). The Annexin V-FITC/PI apoptosis detection Kit was obtained from Bioworld (Louis Park, MN, USA). The transfection reagent VigoFect was from Vigorous (Beijing, China). Hoechst 33342 was from Invitrogen (Carlsbad, CA, USA). All other reagents were local products of analytical grade.