145 2c11 mouse anti cd3 crl 1975 hybridoma cell line

DNCB (2, 4-dinitrochlorobenzene), mite extract, phorbol 12-myristate 13-acetate (PMA), A23187, and carboxyfluoresceinsuccinimidyl ester (CFSE) were purchased from Sigma (St. Louis, MO). FITC-anti-mouse CD4, PerCP cy5.5-anti-mouse IFN-γ, PE-anti-mouse IL-4, and FITC-anti-mouse CD4 were obtained from e-Bioscience (San Diego, CA). A mouse IgE ELISA kit, purified rat anti-mouse IFN-γ, and purified rat anti-mouse IL-12 were obtained from BD Biosciences (San Jose, CA). Mouse anti-CD28, mouse IL-4 ELISA kit, recombinant human IFN-γ, and recombinant human TNF-α were purchased from R&D Systems (Minneapolis, MN). Recombinant mouse IL-4 was obtained from Peprotech (Hamburg, Germany). The 145-2C11 (mouse anti-CD3; CRL-1975) hybridoma cell line was purchased from the ATCC (Manassas, VA). HaCaT keratinocytes were cultured in RPMI 1640 containing 2 mM L-glutamine, antibiotics (100 μg/mL streptomycin, 100 U/mL penicillin), and 10% fetal bovine serum. Cells were incubated at 37°C in a humidified atmosphere of 5% CO₂.

DNCB, mite extract, phorbol 12-myristate 13-acetate (PMA), A23187, and carboxyfluorescein succinimidyl ester (CFSE) were purchased from Sigma (St. Louis, MO). Mouse IgG2a enzyme-linked immunosorbent assay (ELISA) kits, anti-mouse CD4 antibody conjugated with fluorescein isothiocyanate (FITC), anti-mouse interferon (IFN)γ antibody conjugated with PerCP cy5.5, and anti-mouse IL-4 antibody conjugated with phycoerythrin were obtained from eBioscience (San Diego, CA). Rabbit anti-mouse keratin5 antibody was purchased from Abcam (Cambridge, MA) and rabbit anti-mouse p65 antibody was from Cell signaling technology (Beverly, MA). Mouse IgE ELISA kits, purified anti-mouse IFNγ, anti-mouse IL-4, and anti-mouse IL-12 antibodies were purchased from BD Bioscience (San Jose, CA). Anti-mouse CD28 antibody, mouse IFNγ ELISA kits, mouse IL-4 ELISA kits, and recombinant human IFNγ and TNFα were purchased from R&D (Minneapolis, MN). Recombinant mouse IL-4 and IL-12 were obtained from Peprotech (Hamburg, Germany). 145-2C11 (mouse anti-CD3; CRL-1975) hybridoma cell line and HaCaT (human keratinocyte) were obtained from the ATCC (Manassas, VA).