Anti rank

Manufactured by Abcam

Sourced in United Kingdom

Anti-RANK is a laboratory reagent used in research applications. It functions as an antibody that binds to the Receptor Activator of Nuclear Factor Kappa-B (RANK) protein. The core function of Anti-RANK is to facilitate the study and analysis of RANK and its role in cellular processes.

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Lab products found in correlation

Anti il 1β, by Abcam (2 mentions) Nitrocellulose membrane, by Merck Group (1 mentions) Anti tlr2, by Abcam (1 mentions) Anti gapdh, by Abcam (1 mentions) Anti ocn, by Abcam (1 mentions) Anti tlr4, by Abcam (1 mentions) Anti rankl, by Novus Biologicals (1 mentions) Anti tnf α, by Abcam (1 mentions) Anti opg, by Abcam (1 mentions) Bca protein assay kit, by Aspen (1 mentions) Lysis buffer, by Aspen (1 mentions) Anti β catenin, by Cell Signaling Technology (1 mentions) Anti runx2, by Cell Signaling Technology (1 mentions) Anti p p65, by Abcam (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Human soluble rankl, by Thermo Fisher Scientific (1 mentions) M csf, by Thermo Fisher Scientific (1 mentions) Mg132, by Merck Group (1 mentions) γ secretase inhibitor, by Merck Group (1 mentions) C fms, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-RANK antibody (3 citations) Mouse anti-RANK (2 citations)

5 protocols using anti rank

Western Blot Analysis of Osteogenic Markers

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Cells were harvested, lysed with lysis buffer (ASPEN, Wuhan, China), and
centrifuged at 16,200 g for 10 min. Total proteins in the supernatant were
measured using a BCA protein assay kit (ASPEN, Wuhan, China). Total proteins
were extracted from MC3T3-E1 cells. The protein at 30 µg was resolved by 10%
SDS-PAGE gels (ASPEN, Wuhan, China). After electrophoresis, the proteins were
transferred onto nitrocellulose membrane (Millipore, USA). The membranes were
blocked with 5% nonfat milk (ASPEN, Wuhan, China) at room temperature for 1 h.
The samples were probed with anti-GAPDH (1:10,000; Abcam, UK), anti-IL-1β
(1:1000; Abcam, UK), anti-TNF-α (1:500; Abcam, UK), anti-RANK (1:1000; Abcam,
UK), anti-RANKL (1:500; Novusbio, Shanghai, China), anti-OPG (1:1000; Abcam,
UK), anti-OCN (1:500; Abcam, UK), anti-TLR2 (1:1000; Abcam, UK), and anti-TLR4
(1:500; Abcam, UK) Abs. HRP-conjugated goat anti-rabbit IgG (1:10000; ASPEN,
Wuhan, China) was used for detection.

Zhang H., Liu L., Jiang C., Pan K., Deng J, & Wan C. (2019). MMP9 protects against LPS-induced inflammation in osteoblasts. Innate Immunity, 26(4), 259-269.

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Protein Expression Analysis in Cells

Cited 1 time

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Protein quantification was performed. Protein lysate was introduced to SDS-PAGE and subsequently electrotransferred to a polyvinylidene fluoride membrane. Western blotting was carried out as previously reported.^{26 (link)} The antibodies used were listed as follows: anti-RANK (Abcam, ab200369, 1:800), anti-RUNX2 (Cell Signaling Technology, 12486, 1:1 000), anti-ALP (Millipore, 06-942, 1:1 000), anti-pp65 (Abcam, ab76307, 1:5 000), anti-p65 (Active Motif, 39159, 1:1 000), anti-β catenin (Cell Signaling Technology, 4824, 1:200).

Chen X., Zhi X., Wang J, & Su J. (2018). RANKL signaling in bone marrow mesenchymal stem cells negatively regulates osteoblastic bone formation. Bone Research, 6, 34.

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Osteoclastogenesis Regulation Pathway

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Anti-NFATc1 was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-RANK was purchased from Abcam (Cambridge, UK). Anti-Cav-1, cFms and other antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, CA, USA). Human soluble RANKL and M-CSF were purchased from Pepro-Tech (Rocky Hill, NJ, USA). MG132 and γ-secretase inhibitor were purchased from Sigma (St Louis, MO, USA).

Lee Y.D., Yoon S.H., Ji E, & Kim H.H. (2015). Caveolin-1 regulates osteoclast differentiation by suppressing cFms degradation. Experimental & Molecular Medicine, 47(10), e192-.

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Multicolor Immunohistochemistry for Cell Markers

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Antigen retrieval and blocking were performed on the dewaxed slides using the Antigen retrieval reagent (pH 6.0) and Blocking/antibody diluent provided in the Opal Polaris Multicolor Manual IHC Detection Kit (Akoya Biosciences, USA), following the manufacturer’s instructions. In brief, the incubation of each primary antibody was done overnight at 4 °C. The primary antibodies used in this study included anti-CD68 (Abcam, ab31630, USA), anti-TRAP (Abcam, ab216025), anti-RANK (Abcam, ab13918), anti-IL-1β (Abcam, ab9722), anti-NP (Thermo Fisher, USA), anti-ACE2 (Thermo Fisher). Between each incubation of the primary antibody, tyramide signal amplification (TSA) visualization was performed using the Opal Polymer Horseradish peroxidase (HRP) secondary antibody and fluorophores: Opal 520, Opal 570, Opal 620, Opal 690, and DAPI (Akoya Biosciences, USA). The stained slides were imaged using the Vectra Polaris Imaging System (Akoya Biosciences, USA).

Qiao W., Lau H.E., Xie H., Poon V.K., Chan C.C., Chu H., Yuan S., Yuen T.T., Chik K.K., Tsang J.O., Chan C.C., Cai J.P., Luo C., Yuen K.Y., Cheung K.M., Chan J.F, & Yeung K.W. (2022). SARS-CoV-2 infection induces inflammatory bone loss in golden Syrian hamsters. Nature Communications, 13, 2539.

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Immunodetection of Cellular Markers

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The following primary antibodies were used in this study: anti-CD59 [MEM-43] (cat. no. ab9182; Abcam, Cambridge, UK), anti-cyclin D1/FSTL3 (cat. no. bs-0623R; Bioss), anti-osteopontin (cat. no. ab33046; Abcam), anti-NF-κB p105/p50 [E381] (cat. no. ab32360; Abcam), anti-RANK [EPR4740(N)] (cat. no. ab182158; Abcam), anti-RANKL [12A668] (cat. no. ab45039.7; Abcam), and anti-RANK (cat. no. ab222215; Abcam).
The following secondary antibodies were used in this study: goat anti-mouse IgG H&L (Alexa Fluor 488; cat. no. ab150113; Abcam), goat anti-rabbit IgG H&L (Alexa Fluor 488; cat. no. ab150077; Abcam), goat anti-rabbit IgG H&L (horseradish peroxidase (HRP)) preadsorbed (cat. no. ab97080; Abcam), and goat anti-mouse IgG H&L (HRP) preadsorbed (cat. no. ab97040; Abcam).

Yan B., Li Y., Min S., Zhang P., Xu B., Wang Z., Zhang W., Chen J., Luo G, & Liu C. (2020). Effects of the Bone/Bone Marrow Microenvironments on Prostate Cancer Cells and CD59 Expression. BioMed Research International, 2020, 2753414.

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