Cdc 2019 novel coronavirus real time rt pcr assay

Nasopharyngeal swabs or combined oropharyngeal and nares swabs were sent to the Clinical Virology Laboratory at CHLA for testing. The molecular assays used were the CDC 2019-Novel Coronavirus Real-Time RT-PCR assay, the Taqpath COVID-19 RT-PCR assay (Thermo Fisher, Walham, MA), the Xpert Xpress SARS-CoV-2 assay (Cepheid, Sunnyvale, CA), and the Simplexa COVID-19 assay (Diasorin Molecular, Cypress, CA). Total nucleic acid was extracted according to manufacturer’s instructions as outlined in the respective Emergency Use Authorization.^{11 (link)–14} Viral loads (copies/mL) were calculated based on a standard curve generated by testing samples with known viral copy numbers for each assay as previously described.^{11 (link)}

Nasopharyngeal swabs or combined oropharyngeal and nares swabs were sent to the Clinical Virology Laboratory at CHLA for testing. The molecular assays used were the CDC 2019-Novel Coronavirus Real-Time RT-PCR assay, the Taqpath COVID-19 RT-PCR assay (Thermo Fisher, Walham, MA), the Xpert Xpress SARS-CoV-2 assay (Cepheid, Sunnyvale, CA), and the Simplexa COVID-19 assay (Diasorin Molecular, Cypress, CA). Total nucleic acid was extracted according to manufacturer's instructions as outlined in the respective Emergency Use Authorization [[11] , [12] , [13] , [14] ]. Viral loads (copies/mL) were calculated based on a standard curve generated by testing samples with known viral copy numbers for each assay as previously described [11 ].