All chemicals used in this study were purchased from Sigma (St. Louis, MO), unless otherwise noted. Ultrapure LPS, ATP, caspase-1 inhibitor ZVAD-FMK were purchased from Invivogen (San Diego, CA). Mouse IL-1β from PeproTech (Rocky Hill, NJ); human or Mouse IL-1β ELISA kits were from R&D system (Minneapolis, MN) or eBioscience (San Diego, California); Antibodies to Mouse IL-1β and mouse caspase-1 were from R&D Systems or Santa Cruz Biotechnology (Santa Cruz, CA).
Wild type (WT) C57BL/6, caspase-1 KO and ob/ob (B6.Cg-Lepob/J) mice were purchased from the Jackson Laboratory (Bar Harbor, Maine). All mice were maintained at MUSC Hollings animal facility under specific pathogen-free conditions. All animal experiments were approved by the Institutional Animal Care & Use Committee (IACUC) at MUSC, and were conducted in accordance with federal regulations as well as institutional guidelines and regulations on animal studies.
Wild type (WT) C57BL/6, caspase-1 KO and ob/ob (B6.Cg-Lepob/J) mice were purchased from the Jackson Laboratory (Bar Harbor, Maine). All mice were maintained at MUSC Hollings animal facility under specific pathogen-free conditions. All animal experiments were approved by the Institutional Animal Care & Use Committee (IACUC) at MUSC, and were conducted in accordance with federal regulations as well as institutional guidelines and regulations on animal studies.