Cell proliferation was analyzed using a MTT assay (Amresco, Shanghai, China). The cells were then seeded into 96-well plates (4 × 103 cells/well). At the appropriate time after transfection, 20 µL of MTT solution (5 mg/mL) was added to each well. After 3 h, media containing MTT solution were removed and 100 µL DMSO (Sigma-Aldrich, St. Louis, MO, USA) was added to each well. The absorbance at 540 nm was measured with a SoftMax apparatus (Molecular Devices, Sunnyvale, CA, USA). At least three independent experiments were performed to confirm the reproducibility of the results.
Softmax apparatus
Manufactured by Molecular Devices
Sourced in United States
The SoftMax apparatus is a piece of laboratory equipment designed for the measurement and analysis of microplate-based assays. It provides precise and reproducible optical density, absorbance, and fluorescence readings for a variety of biological and chemical applications.
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4 protocols using softmax apparatus
1
Cell Proliferation Assay Using MTT
2
Cell Proliferation Analysis by MTT Assay
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Cell proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Amresco, Shanghai, China). AGS-EBV, SNU-719, or DKK1 over-expressing AGS-EBV cells (
3
MTT Assay for Cell Proliferation
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Cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Cayman, Ann Arbor, MI, USA). The AGS and AGS-EBV cells (4 × 105 cells/well) were seeded into 96-well plates. After the indicated periods following transfection, 20 µL of MTT solution (5 mg/mL) was added to each well. The absorbance at 570 nm was measured with a SoftMax apparatus (Molecular Devices, San Jose, CA, USA) 4 h after adding MTT solution.
4
Cell Proliferation Analysis by MTT Assay
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Cell proliferation was analyzed by using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Amresco, Shanghai, China). AGS cells (3 x103) were seeded in 96-well plates. At a set time following transfection, 20 µl of MTT solution (5 mg/mL) was added to each well. After 4 h, the medium and MTT solution were removed. Then, 100 µl DMSO (Sigma-Aldrich, St. Louis, MO, USA) was added to each well. The absorbance at 595 nm was measured with SoftMax apparatus (Molecular Devices, Sunnyvale, CA, USA) 24 h after adding the DMSO.
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