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Poly lactide co glycolide plga

Manufactured by Merck Group
Sourced in United States

Poly(lactide-co-glycolide) (PLGA) is a biodegradable and biocompatible copolymer that is commonly used in the development of drug delivery systems and tissue engineering applications. It is composed of lactic acid and glycolic acid monomers. The specific properties and performance of PLGA can be tailored by adjusting the ratio of lactic acid to glycolic acid, as well as the molecular weight of the polymer.

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3 protocols using poly lactide co glycolide plga

1

Osteogenic Potential of PLGA/Ag/HA Composites

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Poly(lactide-co-glycolide) (PLGA (glycolide 15 mol%) average molecular weight (Mw) 240000), silver nitrate (AgNO3), and spherical hydroxyapatite (sHA) were purchased from Sigma-Aldrich. Osteoblast (MC3T3-E1) cell line was obtained from Korea Cell Bank. The 5-bromo-2-deoxyuridine (BrdU) and alizarin red staining kits were supplied by Roche Molecular Biochemicals, USA, and Millipore, USA, respectively, whereas fetal bovine serum (FBS) and penicillin G-streptomycin were acquired from Gibco, Japan. All reagents and chemicals in this study were used as received.
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2

Amphotericin B Loaded PLGA Nanoparticles

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Amphotericin B, poly-lactide co-glycolide (PLGA) and polyvinyl alcohol (PVA) were purchased from Sigma–Aldrich, USA. All other chemicals used in this study were of highest purity.
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3

Biomaterial-based 3D Cell Culture Conditions

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Dulbecco Modified Eagle Medium, alpha-MEM, penicillin/ streptomycin, DPBS were obtained from Lonza. Ascorbic acid 2-phosphate and glycerol 2-phosphate disodium salt hydrate were purchased from Sigma. Fetal bovine serum and M199 medium was obtained from Gibco. ECGS/H were from Promocell. VEGF, TGFb 3 were from Peprotech. Poly(lactide-co-glycolide) (PLGA) (50 : 50 Resomer RG 502, RG 505, RG 504, and 85 : 15 PLGA ester terminated M w 50 000-75 000) and tween 20, biotin, chitosan (CS) (from crab shells), polyethylenimine (PEI) (average M w = 800 Da), poly(L-lactic acid) (PLLA) (viscosity B1.0 dL g À1 ) and poly(vinyl acetate) (PVAc) (average M w = 140 kDa), palmitic acid N-hydroxysuccinimide ester (NHS-palmitic acid) and avidin were from Sigma. All other reagents were from Sigma and used without further purification. Analytical grade chloroform, glacial acetic acid, dichloromethane (DCM) were from Thermo Fisher Scientific, UK. Emulsions were generated using a Branson sonifier 250 at 40% max amplification. 1 H nuclear magnetic resonance spectra were recorded on a Bruker AVA500 spectrometer (500 MHz respectively) at 298 K in deuterated solvents.
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