The antimicrobial activity of levofloxacin incorporated into PHB samples prepared by electrospinning was tested against gram-positive bacterium Micrococcus luteus CCM 1569 (ML), gram-negative bacteria Serratia marcescens (SM) CCM 8587 and Escherichia coli (EC) CCM 7359. For the antimicrobial testing of electrospun films loaded with the drug, agar diffusion tests were conducted. All used microorganisms were supplied by the Czech Collection of Microorganisms, Masaryk University, Brno, Czech Republic. Soybean Casein Digest Agar (Tryptone Soya Agar, Himedia Laboratories, Mumbai, India) was used as the nutrient agar. The samples were cut from the PHB electrospun structures loaded with levofloxacin. The samples with a predetermined weight were placed onto the surface of the agar plate containing a microorganism suspension at a concentration of 5 × 105 CFU per mL. The agar plates were incubated for 24 h at 37 °C. Each sample was tested in triplicate and the growth inhibition halo is presented as the average value with the standard deviation.
Soybean casein digest agar tryptone soya agar
Manufactured by HiMedia
Sourced in India
Soybean Casein Digest Agar, also known as Tryptone Soya Agar, is a culture medium used for the growth and enumeration of a wide range of microorganisms. It provides nutrients essential for the cultivation of bacteria, yeasts, and fungi.
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2 protocols using soybean casein digest agar tryptone soya agar
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Antimicrobial Activity of Levofloxacin-Loaded PHB
2
Antibacterial Efficacy of KL^f Scaffold
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For antibacterial testing, two bacterial strains were utilized – Staphylococcus aureus CCM 4516 (ATCC; American Type Culture Collection 6538) and Escherichia coli CCM 4517 (ATCC; American Type Culture Collection 8739). Soybean Casein Digest Agar (Tryptone Soya Agar, HiMedia Laboratories, India) was used as nutrient agar.
To determine whether the amount of KLf was sufficient to induce antibacterial properties in the selected scaffolds, the agar diffusion test with modifications was conducted. The amendment lay in the pouring of samples into agar instead of the placing of flat samples onto the surface of the agar. Samples were poured into the agar containing a bacterial suspension at a concentration of 5 × 105 CFU per mL of agar. The agar plates with inoculated bacteria were incubated at 35 °C for 24 hours. After this incubation period, inhibition zones were measured.
To determine whether the amount of KLf was sufficient to induce antibacterial properties in the selected scaffolds, the agar diffusion test with modifications was conducted. The amendment lay in the pouring of samples into agar instead of the placing of flat samples onto the surface of the agar. Samples were poured into the agar containing a bacterial suspension at a concentration of 5 × 105 CFU per mL of agar. The agar plates with inoculated bacteria were incubated at 35 °C for 24 hours. After this incubation period, inhibition zones were measured.
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