Trans-RSV (Sigma-Aldrich) was solubilized in absolute ethanol (10 mg/mL) and redispersed in saline to a final concentration of 1 mg/mL (in 10% ethanol) immediately before administration. Treatments with RSV or RSV-LNCs at doses of 2.5, 5, or 10 mg/kg were given orally at 1, 4, 6 or 12 h before the LPS challenge. Dexamethasone (5 mg/kg) was given orally 4 h before the LPS challenge. For the vehicle control, the animals were treated with the same amount of ULNCs as in the highest dose of RSV used. The analyses were performed 24 h after the LPS challenge.
Trans rsv
Manufactured by Merck Group
Sourced in United States, Germany
Trans-RSV is a laboratory equipment product designed to detect and analyze respiratory syncytial virus (RSV) in clinical samples. It utilizes a real-time reverse transcription-polymerase chain reaction (RT-PCR) method to identify the presence of RSV genetic material. The product is intended for use in clinical diagnostic laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
Potassium phosphate monobasic, by Merck Group (1 mentions)
2 2 azino bis 3 ethylbenzthiazoline 6 sulfonic acid abts, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Ferulic acid, by Merck Group (1 mentions)
Vanillic acid, by Merck Group (1 mentions)
Phenylacetic acid, by Merck Group (1 mentions)
Sinapic acid, by Merck Group (1 mentions)
Syringic acid, by Merck Group (1 mentions)
Epicatechin, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Thermo Fisher Scientific (1 mentions)
4 hydroxybenzoic acid, by Merck Group (1 mentions)
Taxifolin, by Merck Group (1 mentions)
8 protocols using trans rsv
1
RSV-Loaded Lipid Nanocarriers in LPS-Induced Inflammation
2
Niosomal Formulations for Antioxidant Delivery
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Tw80 was purchased to Riedel-de Haën (Munich, Germany) and Sp80 to Fluka (Buchs, Switzerland). Dod, hydrochloric acid, potassium chloride, sodium hydroxide, potassium phosphate monobasic and potassium persulfate were supplied by Merck (Darmstadt, Germany). 2,2’-Azinobis(3-ethylbenzthiazoline-6-sulfonic acid), (ABTS) ≥ 98%, trans-RSV ≥ 99%, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) ≥ 98% were provided by Sigma Aldrich (St. Louis, MO, USA). Ethanol absolute and methanol HPLC quality were obtained by Sintorgan (Buenos Aires, Argentina). MilliQ water was used in all experiments.
Niosomes formed by the mixture of Tw80 and Sp80 were named Tw80:Sp80 niosomes and that composed by the mixture of Tw80, Sp80 and Dod were called Tw80:Sp80:Dod niosomes.
Niosomes formed by the mixture of Tw80 and Sp80 were named Tw80:Sp80 niosomes and that composed by the mixture of Tw80, Sp80 and Dod were called Tw80:Sp80:Dod niosomes.
3
Trans-RSV Preparation and Handling
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4
Diabetic Retinopathy Treatment in Mice
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Male C57BL6 mice aged 8 weeks were obtained from Gem Pharmatech (Nanjing, China) and kept at Tongji Hospital Animal Center (Wuhan, China). Mice were kept in an animal house under a 12-hour light-dark cycle with food and water ad libitum. Four sets of mice were created: a control group, a control with trans-RSV treated group (RSV), an STZ-induced diabetes mellitus (DM) group, and a DM + RSV group. Twenty mice were included in each group. DM was induced by intraperitoneal injection of STZ (50 mg/kg; Sigma‒Aldrich, St. Louis, MO, USA) for 5 days. After 3 days, mice with blood glucose ≥16.7 mmol/L were considered to be diabetic. Trans-RSV (Sigma‒Aldrich) was administered by gavage at 10 mg/kg body weight/day for 12 weeks. Trans-RSV powder was stored away from light. An appropriate volume of suspension was freshly configured with PBS buffer for each use. After electroretinogram tests, the eyeballs were removed, paraffin embedded, and sectioned or separated on ice. All animal experiments were approved by the Institutional Animal Research Committee of Tongji Medical Center and were conducted in strict accordance with the Guide for the Care and Use of Laboratory Animals (National Institutes of Health, Bethesda, MD, USA).
5
Quantification of Polyphenolic Compounds
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US Pharmacopeia reference standard compounds, including trans-RSV, (+)-catechin, (–)-epicatechin, gallic acid, 3-hydroxytyrosol, isochlorogenic acid, 3, 4-dihydroxyphenylacetic acid, 4-methyl gallic acid, 3, 4-dihydroxyphenylacetic acid, 3-hydroxybenzoic acid, caffeic acid, 4-hydroxybenzoic acid, vanillic acid, dihydromyricetin, syringic acid, resveratrol-3-glucoside, dihydroferulic acid, sinapic acid, taxifolin, ferulic acid, 3-hydrocinnamic acid, phenylacetic acid, and trans-2-hydrocinnamic acid, were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Primary analytical standard (Grade P) compounds, including procyanidin B2, procyanidin C1, quercetin, and cyanidin-3-glucoside, were purchased from ChromaDex (Irvine, CA, USA). The HPLC grade water, acetonitrile (ACN), methanol (MeOH), formic acid (FA), and trifluoroacetic acid (TFA) were obtained from Fisher Scientific Co. (Fair Lawn, NJ, USA).
6
Evaluating Cytotoxicity of Ester Derivatives
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Trans-RSV, dimethyl sulfoxide (DMSO), TMA, chloroform, 2-mercaptoethanol, and (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased directly from Sigma-Aldrich® (Merck KGaA, Darmstadt, Germany). Penicillin–streptomycin and 0.5% trypsin-EDTA were purchased from Gibco (Thermo Fisher Scientific Inc., Waltham, MA, USA). Human liver cancer cell lines (RM60025), C. asparagiforme (BCRC 80850), and B. longum subsp. longum (BCRC 14664) were purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan). ED2 and ED4 were the primary ester derivatives of RBE, and three samples were obtained by this team from the isolation of a previous study [14 (link)].
7
Quantification of trans-Resveratrol by GC-MS
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Hexane, chloroform, ethylchloroformate, trans-RSV, and pinostilbene (internal standard) were purchased from Sigma-Aldrich (Germany). Standard stock solutions were prepared by dissolving trans-RSV and pinostilbene in ethanol. All calibrations were performed by diluting the stock solutions in wine-like matrix (50 mL final volume containing: 150 mg disodium tartrate, 6.5 mL ethanol (13% final concentration), 43.5 mL H2O adjusted to pH 3.3 with an aqueous tartaric acid solution). cis-RSV standard solution was prepared according to Francioso et al. [13 (link)]. Briefly, 30 μg/mL trans-RSV in wine-like matrix were exposed for 2 min to UV light (20 cm from the irradiation source, with a 14.7 W UV-B fluorescent tube emitting at wavelengths of 270−320 nm with a peak at 312 nm). Conversion rate was estimated using GC–MS by comparing trans-RSV peak areas before and after the treatment with UV light.
8
Fabrication of Polymer Nanoparticles
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Poly(ε-caprolactone) (Mn = 14,000 g mol−1), trans-RSV, and PVA (poly(vinyl alcohol), Mw = 30,000–70,000 Da) were supplied by Sigma-Aldrich Química (Madrid, Spain). Dichloromethane and ethanol were purchased from Fluka (Sigma-Aldrich Química). All other reagents were of reagent grade.
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