Antibodies against n cadherin

Manufactured by Cell Signaling Technology

Sourced in United Kingdom, United States

Antibodies against N-cadherin are a type of laboratory reagent used to detect and analyze the presence and distribution of the N-cadherin protein in biological samples. N-cadherin is a cell adhesion molecule that plays a role in cell-cell interactions and tissue organization.

Automatically generated - may contain errors

Lab products found in correlation

E cadherin, by Cell Signaling Technology (2 mentions) Mmp 9, by Cell Signaling Technology (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) E cadherin, by Santa Cruz Biotechnology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Beyotime (1 mentions) Anti grp78, by Abcam (1 mentions) Secondary antibodies conjugated with horseradish peroxidase, by Merck Group (1 mentions) Matrigel basement membrane matrix, by Corning (1 mentions) P gsk3β s9, by Cell Signaling Technology (1 mentions) Enhanced chemi luminescence (ecl), by GE Healthcare (1 mentions) Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions) Sc 48341, by Santa Cruz Biotechnology (1 mentions) Cell counting kit 8 kit, by Beyotime (1 mentions) Bromodeoxyuridine brdu proliferation assay kit, by Merck Group (1 mentions) Chloroquine diphosphate salt c6628, by Merck Group (1 mentions)

3 protocols using antibodies against n cadherin

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in RIPA buffer containing 1mM protease inhibitors PMSF (Beyotime, Shanghai China). Equal amounts of protein were separated and then blotted with antibodies as follows: antibodies against HNF4α (C19, 1:500), E-cadherin (1:500) and β-actin (1:500) from Santa Cruz Biotechnology (Santa Cruz, USA), anti-GRP78 (1:1000) from Abcam (Cambridge, UK), antibodies against N-cadherin (1:1000), MMP9 (1:1000) and Bcl-2 (1:1000) from Cell Signaling Technology (Beverly, MA, USA) and HRP-conjugated species-specific secondary antibodies (1:2000 dilution, ProteinTech Group, Wuhan, China). After enhanced chemiluminescence reaction (Tiangen, Beijing, China), relative amounts of protein were quantified using Image Gel-Pro analyzer software (Media Cybernetics, Florida, USA).

Jiang Q., Sun Y., Guo Z., Chen R., Ma S., Fu M., Zhu H., Ning Q., Lei P, & Shen G. (2018). IL-23 enhances the malignant properties of hepatoma cells by attenuation of HNF4α. Oncotarget, 9(47), 28309-28321.

+ Open protocol

+ Expand

Antibody-based Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents were of analytical grade and were purchased from Sigma (St. Louis, MO, USA) unless otherwise specified. Cell culture dishes and flasks were from TPP (Switzerland). ECL was from GE Healthcare (Wauwatosa, WI, USA). Antibodies against PRDX6 were from Mybiosource (Cambridge, UK). Antibodies against N-cadherin, caspase-3, E-cadherin, PCNA, GSK3β, pGSK3β^S9, and pAKT^S473 were purchased from Cell Signaling (Danvers, MA, USA). Antibodies against AKT and actin were from Santa Cruz (Dallas, TX, USA). HA-flag antibody was from Thermo Fisher Scientific (Waltham, MA, USA), and complex I antibody and secondary antibodies conjugated with horseradish peroxidase were from Sigma. Secondary antibodies conjugated with Alexa Fluor 488 and 647 fluorophores and Alexa Fluor 488 phalloidin were from Abcam (Cambridge, UK), and Matrigel^® Matrix basement membrane was from Corning (New York, NY, USA).

Lagal D.J., López-Grueso M.J., Pedrajas J.R., Leto T.L., Bárcena J.A., Requejo-Aguilar R, & Padilla C.A. (2023). Loss of PRDX6 Aborts Proliferative and Migratory Signaling in Hepatocarcinoma Cell Lines. Antioxidants, 12(6), 1153.

+ Open protocol

+ Expand

Autophagy and Epithelial-Mesenchymal Transition

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies against SQSTM1 (PB0458, 1:400) was obtained from Boster Biological Technology Co. Ltd. Antibody against MFN1 (ab107129), LC3B (ab48394), Pink (ab23707), Parkin (ab77924) and Snail (ab53519) were purchased from Abcam. Antibodies against BECN-1 (sc-48341) and Fis 1(sc-376469) were purchased from Santa Cruz Biotechnology, Inc. Antibodies against N-cadherin (#13116) and E-cadherin (#14472) were obtained from Cell Signalling Technology. The Cell Counting Kit-8 kit (C0037) was provided by Beyotime Institute of Biotechnology. Bromodeoxyuridine (BrdU) proliferation assay kit (2750) was purchased from Millipore Corporation. The immunocytochemistry detection kits (SPN-9001) obtained from ZSGB-BIO. Chloroquine diphosphate salt (C6628) was purchased Sigma. mRFP-eGFP-LC3 plasmid was obtained from Hanbio Biotechnology Co. Ltd (Shanghai, China). Enhanced chemiluminescence (ECL, RPN2236) reagents were from Amersham International. All other reagents were from common commercial sources.

Liu X., Feng C., Wei G., Kong W., Meng H., Du Y, & Li J. (2020). Mitofusin1 Is a Major Mediator in Glucose-Induced Epithelial-to-Mesenchymal Transition in Lung Adenocarcinoma Cells. OncoTargets and therapy, 13, 3511-3523.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!